3WTF

Structure of PAXX

3WTF の概要

• エントリーDOI: 10.2210/pdb3wtf/pdb
• 関連するPDBエントリー: 3WTD
• 分子名称: Uncharacterized protein C9orf142 (2 entities in total)
• 機能のキーワード: dna repair, scaffold, immune system
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43615.26

構造登録者

Ochi, T.,Blundell, T.L. (登録日: 2014-04-09, 公開日: 2015-01-21, 最終更新日: 2023-11-08)

主引用文献

Ochi, T.,Blackford, A.N.,Coates, J.,Jhujh, S.,Mehmood, S.,Tamura, N.,Travers, J.,Wu, Q.,Draviam, V.M.,Robinson, C.V.,Blundell, T.L.,Jackson, S.P.
DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair.
Science, 347:185-188, 2015

PubMed Abstract: XRCC4 and XLF are two structurally related proteins that function in DNA double-strand break (DSB) repair. Here, we identify human PAXX (PAralog of XRCC4 and XLF, also called C9orf142) as a new XRCC4 superfamily member and show that its crystal structure resembles that of XRCC4. PAXX interacts directly with the DSB-repair protein Ku and is recruited to DNA-damage sites in cells. Using RNA interference and CRISPR-Cas9 to generate PAXX(-/-) cells, we demonstrate that PAXX functions with XRCC4 and XLF to mediate DSB repair and cell survival in response to DSB-inducing agents. Finally, we reveal that PAXX promotes Ku-dependent DNA ligation in vitro and assembly of core nonhomologous end-joining (NHEJ) factors on damaged chromatin in cells. These findings identify PAXX as a new component of the NHEJ machinery.

PubMed: 25574025
DOI: 10.1126/science.1261971

実験手法

X-RAY DIFFRACTION (3.451 Å)