3WTD

Structure of PAXX

Summary for 3WTD

• Entry DOI: 10.2210/pdb3wtd/pdb
• Related: 3WTF
• Descriptor: Uncharacterized protein C9orf142 (2 entities in total)
• Functional Keywords: dna repair, scaffold, nuclear protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 35706.32

Authors

Ochi, T.,Blundell, T.L. (deposition date: 2014-04-09, release date: 2015-01-21, Last modification date: 2024-03-20)

Primary citation

Ochi, T.,Blackford, A.N.,Coates, J.,Jhujh, S.,Mehmood, S.,Tamura, N.,Travers, J.,Wu, Q.,Draviam, V.M.,Robinson, C.V.,Blundell, T.L.,Jackson, S.P.
DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair.
Science, 347:185-188, 2015

PubMed Abstract: XRCC4 and XLF are two structurally related proteins that function in DNA double-strand break (DSB) repair. Here, we identify human PAXX (PAralog of XRCC4 and XLF, also called C9orf142) as a new XRCC4 superfamily member and show that its crystal structure resembles that of XRCC4. PAXX interacts directly with the DSB-repair protein Ku and is recruited to DNA-damage sites in cells. Using RNA interference and CRISPR-Cas9 to generate PAXX(-/-) cells, we demonstrate that PAXX functions with XRCC4 and XLF to mediate DSB repair and cell survival in response to DSB-inducing agents. Finally, we reveal that PAXX promotes Ku-dependent DNA ligation in vitro and assembly of core nonhomologous end-joining (NHEJ) factors on damaged chromatin in cells. These findings identify PAXX as a new component of the NHEJ machinery.

PubMed: 25574025
DOI: 10.1126/science.1261971

Experimental method

X-RAY DIFFRACTION (2.35 Å)