3WSF

Oxidized HcgD from Methanocaldococcus jannaschii with citrate

3WSF の概要

• エントリーDOI: 10.2210/pdb3wsf/pdb
• 関連するPDBエントリー: 3WSD 3WSE 3WSG 3WSH 3WSI
• 分子名称: Putative GTP cyclohydrolase 1 type 2, FE (III) ION, CITRIC ACID, ... (4 entities in total)
• 機能のキーワード: nif3-related protein, metal binding protein
• 由来する生物種: Methanocaldococcus jannaschii
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 171015.13

構造登録者

Fujishiro, T.,Ermler, U.,Shima, S. (登録日: 2014-03-13, 公開日: 2014-08-27, 最終更新日: 2023-11-08)

主引用文献

Fujishiro, T.,Ermler, U.,Shima, S.
A possible iron delivery function of the dinuclear iron center of HcgD in [Fe]-hydrogenase cofactor biosynthesis
Febs Lett., 588:2789-2793, 2014

PubMed Abstract: HcgD, a homolog of the ubiquitous Nif3-like protein family, is found in a gene cluster involved in the biosynthesis of the iron-guanylylpyridinol (FeGP) cofactor of [Fe]-hydrogenase. The presented crystal structure and biochemical analyses indicated that HcgD has a dinuclear iron-center, which provides a pronounced binding site for anionic ligands. HcgD contains a stronger and a weaker bound iron; the latter being removable by chelating reagents preferentially in the oxidized state. Therefore, we propose HcgD as an iron chaperone in FeGP cofactor biosynthesis, which might also stimulate investigations on the functionally unknown but physiologically important eukaryotic Nif3-like protein family members.

PubMed: 24931373
DOI: 10.1016/j.febslet.2014.05.059

実験手法

X-RAY DIFFRACTION (2 Å)