3WQV

Crystal structure of Ostrinia furnacalis Group I chitinase catalytic domain in complex with a(GlcN)5

3WQV の概要

• エントリーDOI: 10.2210/pdb3wqv/pdb
• 関連するPDBエントリー: 3WQW
• 分子名称: Chitinase, 2-amino-2-deoxy-beta-D-glucopyranose-(1-4)-2-amino-2-deoxy-beta-D-glucopyranose-(1-4)-2-amino-2-deoxy-beta-D-glucopyranose-(1-4)-2-amino-2-deoxy-beta-D-glucopyranose-(1-4)-2-amino-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: family-18 chitinases, inhibition, deacetylated chitooligosaccharides, hydrolase
• 由来する生物種: Ostrinia furnacalis (Asian corn borer)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46500.13

構造登録者

Chen, L.,Zhou, Y.,Yang, Q. (登録日: 2014-02-03, 公開日: 2014-05-28, 最終更新日: 2024-10-16)

主引用文献

Chen, L.,Zhou, Y.,Qu, M.,Zhao, Y.,Yang, Q.
Fully deacetylated chitooligosaccharides act as efficient glycoside hydrolase family 18 chitinase inhibitors.
J.Biol.Chem., 289:17932-17940, 2014

PubMed Abstract: Small molecule inhibitors against chitinases have potential applications as pesticides, fungicides, and antiasthmatics. Here, we report that a series of fully deacetylated chitooligosaccharides (GlcN)2-7 can act as inhibitors against the insect chitinase OfChtI, the human chitinase HsCht, and the bacterial chitinases SmChiA and SmChiB with IC50 values at micromolar to millimolar levels. The injection of mixed (GlcN)2-7 into the fifth instar larvae of the insect Ostrinia furnacalis resulted in 85% of the larvae being arrested at the larval stage and death after 10 days, also suggesting that (GlcN)2-7 might inhibit OfChtI in vivo. Crystal structures of the catalytic domain of OfChtI (OfChtI-CAD) complexed with (GlcN)5,6 were obtained at resolutions of 2.0 Å. These structures, together with mutagenesis and thermodynamic analysis, suggested that the inhibition was strongly related to the interaction between the -1 GlcN residue of the inhibitor and the catalytic Glu(148) of the enzyme. Structure-based comparison showed that the fully deacetylated chitooligosaccharides mimic the substrate chitooligosaccharides by binding to the active cleft. This work first reports the inhibitory activity and proposed inhibitory mechanism of fully deacetylated chitooligosaccharides. Because the fully deacetylated chitooligosaccharides can be easily derived from chitin, one of the most abundant materials in nature, this work also provides a platform for developing eco-friendly inhibitors against chitinases.

PubMed: 24828498
DOI: 10.1074/jbc.M114.564534

実験手法

X-RAY DIFFRACTION (2.043 Å)