3WQ7

New crystal form of the hyperthermophilic family 12 endo-cellulase from Pyrococcus furiosus

3WQ7 の概要

• エントリーDOI: 10.2210/pdb3wq7/pdb
• 関連するPDBエントリー: 3VGI 3WQ0 3WQ1
• 分子名称: Endoglucanase A, CALCIUM ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: beta-jelly roll, glycoside hydrolase, hydrolase
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 73664.97

構造登録者

Kataoka, M.,Ishikawa, K. (登録日: 2014-01-23, 公開日: 2014-07-02, 最終更新日: 2024-03-20)

主引用文献

Kataoka, M.,Ishikawa, K.
A new crystal form of a hyperthermophilic endocellulase.
Acta Crystallogr.,Sect.F, 70:878-883, 2014

PubMed Abstract: The hyperthermophilic glycoside hydrolase family endocellulase 12 from the archaeon Pyrococcus furiosus (EGPf; Gene ID PF0854; EC 3.2.1.4) catalyzes the hydrolytic cleavage of the β-1,4-glucosidic linkage in β-glucan in lignocellulose biomass. A crystal of EGPf was previously prepared at pH 9.0 and its structure was determined at an atomic resolution of 1.07 Å. This article reports the crystallization of EGPf at the more physiologically relevant pH of 5.5. Structure determination showed that this new crystal form has the symmetry of space group C2. Two molecules of the enzyme are observed in the asymmetric unit. Crystal packing is weak at pH 5.5 owing to two flexible interfaces between symmetry-related molecules. Comparison of the EGPf structures obtained at pH 9.0 and pH 5.5 reveals a significant conformational difference at the active centre and in the surface loops. The interfaces in the vicinity of the flexible surface loops impact the quality of the EGPf crystal.

PubMed: 25005081
DOI: 10.1107/S2053230X14010930

実験手法

X-RAY DIFFRACTION (1.68 Å)