3WNP

D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose

Summary for 3WNP

• Entry DOI: 10.2210/pdb3wnp/pdb
• Related: 3WNK 3WNL 3WNM 3WNN 3WNO
• Descriptor: Cycloisomaltooligosaccharide glucanotransferase, alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-beta-D-glucopyranose, CALCIUM ION, ... (7 entities in total)
• Functional Keywords: c2 type immunoglobulin fold, (beta/alpha)8-barrel, beta-jelly roll, greek key, glycoside hydrolase, alpha-1, 6-glucan, transferase
• Biological source: Bacillus circulans
• Total number of polymer chains: 2
• Total formula weight: 161174.80

Authors

Suzuki, R.,Suzuki, N.,Fujimoto, Z.,Momma, M.,Kimura, K.,Kitamura, S.,Kimura, A.,Funane, K. (deposition date: 2013-12-10, release date: 2014-02-05, Last modification date: 2023-11-08)

• Primary citation: Suzuki, R.,Suzuki, N.,Fujimoto, Z.,Momma, M.,Kimura, K.,Kitamura, S.,Kimura, A.,Funane, K.; Molecular engineering of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040: structural determinants for the reaction product size and reactivity.; Biochem.J., 467:259-270, 2015; PubMed: 25649478; DOI: 10.1042/BJ20140860

Experimental method

X-RAY DIFFRACTION (2.8 Å)