3WMU

The structure of an anti-cancer lectin mytilec apo-form from the mussel Mytilus galloprovincialis

3WMU の概要

• エントリーDOI: 10.2210/pdb3wmu/pdb
• 関連するPDBエントリー: 3WMV
• 分子名称: Lectin, GLYCEROL (3 entities in total)
• 機能のキーワード: sugar binding protein, lectin, carbohydrate
• 由来する生物種: Mytilus galloprovincialis (Mediterranean mussel)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34943.79

構造登録者

Terada, D.,Kawai, F.,Noguchi, H.,Unzai, S.,Park, S.-Y.,Ozeki, Y.,Tame, J.R.H. (登録日: 2013-11-27, 公開日: 2014-12-03, 最終更新日: 2024-03-20)

主引用文献

Terada, D.,Kawai, F.,Noguchi, H.,Unzai, S.,Hasan, I.,Fujii, Y.,Park, S.-Y.,Ozeki, Y.,Tame, J.R.H.
Crystal structure of MytiLec, a galactose-binding lectin from the mussel Mytilus galloprovincialis with cytotoxicity against certain cancer cell types
Sci Rep, 6:28344-28344, 2016

PubMed Abstract: MytiLec is a lectin, isolated from bivalves, with cytotoxic activity against cancer cell lines that express globotriaosyl ceramide, Galα(1,4)Galβ(1,4)Glcα1-Cer, on the cell surface. Functional analysis shows that the protein binds to the disaccharide melibiose, Galα(1,6)Glc, and the trisaccharide globotriose, Galα(1,4)Galβ(1,4)Glc. Recombinant MytiLec expressed in bacteria showed the same haemagglutinating and cytotoxic activity against Burkitt's lymphoma (Raji) cells as the native form. The crystal structure has been determined to atomic resolution, in the presence and absence of ligands, showing the protein to be a member of the β-trefoil family, but with a mode of ligand binding unique to a small group of related trefoil lectins. Each of the three pseudo-equivalent binding sites within the monomer shows ligand binding, and the protein forms a tight dimer in solution. An engineered monomer mutant lost all cytotoxic activity against Raji cells, but retained some haemagglutination activity, showing that the quaternary structure of the protein is important for its cellular effects.

PubMed: 27321048
DOI: 10.1038/srep28344

実験手法

X-RAY DIFFRACTION (1.1 Å)