3WLU

Crystal Structure of human galectin-9 NCRD with Selenolactose

3WLU の概要

• エントリーDOI: 10.2210/pdb3wlu/pdb
• 関連するPDBエントリー: 2EAK
• 分子名称: Galectin-9, 2-(trimethylsilyl)ethyl 4-O-beta-D-galactopyranosyl-6-Se-methyl-6-seleno-beta-D-glucopyranoside (3 entities in total)
• 機能のキーワード: selenolactose, beta sandwich, galectin, carbohydrate binding, sugar binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm : O00182
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 65765.38

構造登録者

Makyio, H.,Suzuki, T.,Ando, H.,Yamada, Y.,Ishida, H.,Kiso, M.,Wakatsuki, S.,Kato, R. (登録日: 2013-11-14, 公開日: 2014-11-05, 最終更新日: 2024-03-20)

主引用文献

Suzuki, T.,Makyio, H.,Ando, H.,Komura, N.,Menjo, M.,Yamada, Y.,Imamura, A.,Ishida, H.,Wakatsuki, S.,Kato, R.,Kiso, M.
Expanded potential of seleno-carbohydrates as a molecular tool for X-ray structural determination of a carbohydrate-protein complex with single/multi-wavelength anomalous dispersion phasing
Bioorg.Med.Chem., 22:2090-2101, 2014

PubMed Abstract: Seleno-lactoses have been successfully synthesized as candidates for mimicking carbohydrate ligands for human galectin-9 N-terminal carbohydrate recognition domain (NCRD). Selenium was introduced into the mono- or di-saccharides using p-methylselenobenzoic anhydride (Tol2Se) as a novel selenating reagent. The TolSe-substituted monosaccharides were converted into selenoglycosyl donors or acceptors, which were reacted with coupling partners to afford seleno-lactoses. The seleno-lactoses were converted to the target compounds. The structure of human galectin-9 NCRD co-crystallized with 6-MeSe-lactose was determined with single/multi-wavelength anomalous dispersion (SAD/MAD) phasing and was similar to that of the co-crystal with natural lactose.

PubMed: 24631362
DOI: 10.1016/j.bmc.2014.02.023

実験手法

X-RAY DIFFRACTION (1.4 Å)