3WKL

The periplasmic PDZ tandem fragment of the RseP homologue from Aquifex aeolicus

3WKL の概要

• エントリーDOI: 10.2210/pdb3wkl/pdb
• 関連するPDBエントリー: 3WKM
• 分子名称: Putative Zinc Metalloprotease AQ_1964 (1 entity in total)
• 機能のキーワード: pdz domain, hydrolase
• 由来する生物種: AQUIFEX AEOLICUS
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein (By similarity): O67776
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19997.46

構造登録者

Nogi, T.,Tabata, S.,Tamura-kawakami, K.,Takagi, J. (登録日: 2013-10-28, 公開日: 2013-12-18, 最終更新日: 2023-11-08)

主引用文献

Hizukuri, Y.,Oda, T.,Tabata, S.,Tamura-kawakami, K.,Oi, R.,Sato, M.,Takagi, J.,Akiyama, Y.,Nogi, T.
A Structure-Based Model of Substrate Discrimination by a Noncanonical PDZ Tandem in the Intramembrane-Cleaving Protease RseP
Structure, 22:326-336, 2013

PubMed Abstract: During the extracytoplasmic stress response in Escherichia coli, the intramembrane protease RseP cleaves the anti-σ(E) protein RseA only after the membrane-anchored protease DegS truncates the periplasmic part of RseA that suppresses the action of RseP. Here we analyzed the three-dimensional structure of the two tandemly arranged PSD-95/Dlg/ZO-1 (PDZ) domains (PDZ tandem) present in the periplasmic region of RseP and revealed that the two putative ligand-binding grooves constitute a single pocket-like structure that would lie just above the active center sequestrated within the membrane. Complete removal of the PDZ tandem from RseP led to the intramembrane cleavage of RseA without prior truncation by DegS. Furthermore, mutations expected to destabilize the tertiary structure of the PDZ tandem also caused the deregulation of the sequential cleavage. These observations suggest that the PDZ tandem serves as a size-exclusion filter to accommodate the truncated form of RseA into the active center.

PubMed: 24389025
DOI: 10.1016/j.str.2013.12.003

実験手法

X-RAY DIFFRACTION (2.8 Å)