3WJ9

Crystal structure of the eukaryotic initiation factor

3WJ9 の概要

• エントリーDOI: 10.2210/pdb3wj9/pdb
• 分子名称: Eukaryotic translation initiation factor 2A (2 entities in total)
• 機能のキーワード: beta-propeller, translation
• 由来する生物種: Schizosaccharomyces pombe (Fission yeast)
• 細胞内の位置: Cytoplasm (By similarity): O74965
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 92293.97

構造登録者

Kashiwagi, K.,Ito, T.,Yokoyama, S. (登録日: 2013-10-07, 公開日: 2014-03-12, 最終更新日: 2024-10-09)

主引用文献

Kashiwagi, K.,Ito, T.,Yokoyama, S.
Crystal structure of the eukaryotic translation initiation factor 2A from Schizosaccharomyces pombe.
J Struct Funct Genomics, 15:125-130, 2014

PubMed Abstract: The eukaryotic translation initiation factor 2A (eIF2A) was identified as a factor that stimulates the binding of methionylated initiator tRNA (Met-tRNA i (Met) ) to the 40S ribosomal subunit, but its physiological role remains poorly defined. Recently, eIF2A was shown to be involved in unconventional translation initiation from CUG codons and in viral protein synthesis under stress conditions where eIF2 is inactivated. We determined the crystal structure of the WD-repeat domain of Schizosaccharomyces pombe eIF2A at 2.5 Å resolution. The structure adopts a novel nine-bladed β-propeller fold. In contrast to the usual β-propeller proteins, the central channel of the molecule has the narrower opening on the bottom of the protein and the wider opening on the top. Highly conserved residues are concentrated in the positively-charged top face, suggesting the importance of this face for interactions with nucleic acids or other initiation factors.

PubMed: 24569939
DOI: 10.1007/s10969-014-9177-y

実験手法

X-RAY DIFFRACTION (2.506 Å)