3WHN

Hemerythrin-like domain of DcrH I119H mutant (met)

3WHN の概要

• エントリーDOI: 10.2210/pdb3whn/pdb
• 関連するPDBエントリー: 3AGT 3AGU 3WAQ
• 分子名称: Hemerythrin-like domain protein DcrH, CHLORO DIIRON-OXO MOIETY, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: metal-binding, helix bundle, oxygen sensor, metal binding protein
• 由来する生物種: Desulfovibrio vulgaris
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32772.98

構造登録者

Okamoto, Y.,Onoda, A.,Sugimoto, H.,Takano, Y.,Hirota, S.,Kurtz Jr., D.M.,Shiro, Y.,Hayashi, T. (登録日: 2013-08-29, 公開日: 2014-02-26, 最終更新日: 2023-11-08)

主引用文献

Okamoto, Y.,Onoda, A.,Sugimoto, H.,Takano, Y.,Hirota, S.,Kurtz Jr., D.M.,Shiro, Y.,Hayashi, T.
H2O2-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin.
Chem.Commun.(Camb.), 50:3421-3423, 2014

PubMed Abstract: The O2-binding carboxylate-bridged diiron site in DcrH-Hr was engineered in an effort to perform the H2O2-dependent oxidation of external substrates. A His residue was introduced near the diiron site in place of a conserved residue, Ile119. The I119H variant promotes the oxidation of guaiacol and 1,4-cyclohexadiene upon addition of H2O2.

PubMed: 24400317
DOI: 10.1039/c3cc48108e

実験手法

X-RAY DIFFRACTION (1.9 Å)