3WHM

Structure of Hemoglobin Complex with 18-crown-6

3WHM の概要

• エントリーDOI: 10.2210/pdb3whm/pdb
• 関連するPDBエントリー: 3WH0
• 分子名称: Hemoglobin subunit alpha, Hemoglobin subunit beta, PROTOPORPHYRIN IX CONTAINING FE, ... (6 entities in total)
• 機能のキーワード: 18-crown-6, heme-binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 65964.62

構造登録者

Lee, C.C.,Lin, L.L.,Wang, A.H.J. (登録日: 2013-08-27, 公開日: 2014-10-15, 最終更新日: 2023-11-08)

主引用文献

Lee, C.C.,Maestre-Reyna, M.,Hsu, K.C.,Wang, H.C.,Liu, C.I.,Jeng, W.Y.,Lin, L.L.,Wood, R.,Chou, C.C.,Yang, J.M.,Wang, A.H.
Crowning proteins: modulating the protein surface properties using crown ethers.
Angew.Chem.Int.Ed.Engl., 53:13054-13058, 2014

PubMed Abstract: Crown ethers are small, cyclic polyethers that have found wide-spread use in phase-transfer catalysis and, to a certain degree, in protein chemistry. Crown ethers readily bind metallic and organic cations, including positively charged amino acid side chains. We elucidated the crystal structures of several protein-crown ether co-crystals grown in the presence of 18-crown-6. We then employed biophysical methods and molecular dynamics simulations to compare these complexes with the corresponding apoproteins and with similar complexes with ring-shaped low-molecular-weight polyethylene glycols. Our studies show that crown ethers can modify protein surface behavior dramatically by stabilizing either intra- or intermolecular interactions. Consequently, we propose that crown ethers can be used to modulate a wide variety of protein surface behaviors, such as oligomerization, domain-domain interactions, stabilization in organic solvents, and crystallization.

PubMed: 25287606
DOI: 10.1002/anie.201405664

実験手法

X-RAY DIFFRACTION (1.85 Å)