3WGI

Crystal structure of RSP in complex with beta-NAD+ and operator DNA

3WGI の概要

• エントリーDOI: 10.2210/pdb3wgi/pdb
• 関連するPDBエントリー: 3WG9 3WGG 3WGH
• 分子名称: Redox-sensing transcriptional repressor rex, DNA (5'-D(*TP*AP*GP*AP*TP*TP*GP*TP*TP*AP*AP*TP*CP*GP*AP*TP*TP*AP*AP*CP*AP*AP*TP*C)-3'), NICOTINAMIDE-ADENINE-DINUCLEOTIDE (ACIDIC FORM) (3 entities in total)
• 機能のキーワード: winged helix, rossmann fold, transcription repressor, transcription-dna complex, transcription/dna
• 由来する生物種: Thermoanaerobacter ethanolicus
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 134018.02

構造登録者

Zheng, Y.,Ko, T.-P.,Guo, R.-T. (登録日: 2013-08-05, 公開日: 2014-08-13, 最終更新日: 2023-11-08)

主引用文献

Zheng, Y.,Ko, T.-P.,Sun, H.,Huang, C.-H.,Pei, J.,Qiu, R.,Wang, A.H.-J.,Wiegel, J.,Shao, W.,Guo, R.-T.
Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes.
J.Struct.Biol., 188:195-204, 2014

PubMed Abstract: The Rex-family repressors sense redox levels by alternative binding to NADH or NAD(+). Unlike other Rex proteins that regulate aerobic respiration, RSP controls ethanol fermentation in the obligate anaerobe Thermoanaerobacter ethanolicus JW200(T). It is also found in other anaerobic microorganisms. Here we present the crystal structures of apo-RSP, RSP/NADH and RSP/NAD(+)/DNA, which are the first structures of Rex-family members from an obligate anaerobe. RSP functions as a homodimer. It assumes an open conformation when bound to the operator DNA and a closed conformation when not DNA-bound. The DNA binds to the N-terminal winged-helix domain and the dinucleotide, either reduced or oxidized, binds to the C-terminal Rossmann-fold domain. The two distinct orientations of nicotinamide ring, anti in NADH and syn in NAD(+), give rise to two sets of protein-ligand interactions. Consequently, NADH binding makes RSP into a closed conformation, which does not bind to DNA. Both the conserved residues and the DNA specificity of RSP show a number of variations from those of the aerobic Rex, reflecting different structural bases for redox-sensing by the anaerobic and aerobic Rex-family members.

PubMed: 25463021
DOI: 10.1016/j.jsb.2014.11.001

実験手法

X-RAY DIFFRACTION (3.25 Å)