3WGG

Crystal structure of RSP in complex with alpha-NAD+

3WGG の概要

• エントリーDOI: 10.2210/pdb3wgg/pdb
• 関連するPDBエントリー: 3WG9 3WGH 3WGI
• 分子名称: Redox-sensing transcriptional repressor rex, alpha-Diphosphopyridine nucleotide, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: winged helix, rossmann fold, transcription repressor, transcription
• 由来する生物種: Thermoanaerobacter ethanolicus
• 細胞内の位置: Cytoplasm : D5KM69
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52851.79

構造登録者

Zheng, Y.,Ko, T.-P.,Guo, R.-T. (登録日: 2013-08-05, 公開日: 2014-08-13, 最終更新日: 2023-11-08)

主引用文献

Zheng, Y.,Ko, T.-P.,Yang, Y.,Shao, W.,Guo, R.-T.
Binding mode of the oxidized alpha-anomer of NAD(+) to RSP, a Rex-family repressor
Biochem.Biophys.Res.Commun., 456:733-736, 2015

PubMed Abstract: The Rex-family repressors sense redox levels by alternative binding to NADH or NAD(+). RSP is the homologue of Rex in Thermoanaerobacter ethanolicus JW200(T) and regulates ethanol fermentation in this obligate anaerobe. The dimeric repressor binds to DNA by an open conformation. The crystal structure of RSP/α-NAD(+) complex shows a different set of ligand interactions mainly due to the unique configuration of the nicotinamide moiety. The positively charged ring is covered by the Tyr102 side chain and interacts with a sulfate ion adjacent to the N-terminus of helix α8. Consequently, the RSP dimer may be locked in a closed conformation that does not bind to DNA. However, α-NAD(+) does not show a higher affinity to RSP than β-NAD(+). It has to be improved for possible use as an effector in modulating the repressor.

PubMed: 25527330
DOI: 10.1016/j.bbrc.2014.12.049

実験手法

X-RAY DIFFRACTION (2.1 Å)