3WG4

Crystal structure of Agrocybe cylindracea galectin mutant (N46A) with blood type A antigen tetraose

3WG4 の概要

• エントリーDOI: 10.2210/pdb3wg4/pdb
• 関連するPDBエントリー: 3WG1 3WG2 3WG3
• 関連するBIRD辞書のPRD_ID: PRD_900132
• 分子名称: Galactoside-binding lectin, alpha-L-fucopyranose-(1-2)-[2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-3)]beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: galectin, galactose binding, sugar binding protein
• 由来する生物種: Agrocybe cylindracea
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39493.64

構造登録者

Kuwabara, N.,Hu, D.,Tateno, H.,Makio, H.,Hirabayashi, J.,Kato, R. (登録日: 2013-07-25, 公開日: 2013-11-27, 最終更新日: 2023-11-08)

主引用文献

Kuwabara, N.,Hu, D.,Tateno, H.,Makio, H.,Hirabayashi, J.,Kato, R.
Conformational change of a unique sequence in a fungal galectin from Agrocybe cylindracea controls glycan ligand-binding specificity.
Febs Lett., 587:3620-3625, 2013

PubMed Abstract: A fungal galectin from Agrocybe cylindracea (ACG) exhibits broad binding specificity for β-galactose-containing glycans. We determined the crystal structures of wild-type ACG and the N46A mutant, with and without glycan ligands. From these structures and a saccharide-binding analysis of the N46A mutant, we revealed that a conformational change of a unique insertion sequence containing Asn46 provides two binding modes for ACG, and thereby confers broad binding specificity. We propose that the unique sequence provides these two distinct glycan-binding modes by an induced-fit mechanism.

PubMed: 24036446
DOI: 10.1016/j.febslet.2013.08.046

実験手法

X-RAY DIFFRACTION (1.6 Å)