3WG2

Crystal structure of Agrocybe cylindracea galectin mutant (N46A)

3WG2 の概要

• エントリーDOI: 10.2210/pdb3wg2/pdb
• 関連するPDBエントリー: 3WG1 3WG3 3WG4
• 分子名称: Galactoside-binding lectin, TRIETHYLENE GLYCOL (3 entities in total)
• 機能のキーワード: galectin, galactose binding, sugar binding protein
• 由来する生物種: Agrocybe aegerita
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38072.33

構造登録者

Kuwabara, N.,Hu, D.,Tateno, H.,Makio, H.,Hirabayashi, J.,Kato, R. (登録日: 2013-07-25, 公開日: 2013-11-27, 最終更新日: 2023-11-08)

主引用文献

Kuwabara, N.,Hu, D.,Tateno, H.,Makyio, H.,Hirabayashi, J.,Kato, R.
Conformational change of a unique sequence in a fungal galectin from Agrocybe cylindracea controls glycan ligand-binding specificity.
Febs Lett., 587:3620-3625, 2013

PubMed Abstract: A fungal galectin from Agrocybe cylindracea (ACG) exhibits broad binding specificity for β-galactose-containing glycans. We determined the crystal structures of wild-type ACG and the N46A mutant, with and without glycan ligands. From these structures and a saccharide-binding analysis of the N46A mutant, we revealed that a conformational change of a unique insertion sequence containing Asn46 provides two binding modes for ACG, and thereby confers broad binding specificity. We propose that the unique sequence provides these two distinct glycan-binding modes by an induced-fit mechanism.

PubMed: 24036446
DOI: 10.1016/j.febslet.2013.08.046

実験手法

X-RAY DIFFRACTION (2.2 Å)