3WDF

Staphylococcus aureus UDG

3WDF の概要

• エントリーDOI: 10.2210/pdb3wdf/pdb
• 関連するPDBエントリー: 3WDG
• 分子名称: Uracil-DNA glycosylase (2 entities in total)
• 機能のキーワード: uracil-dna glycosylase, hydrolase
• 由来する生物種: Staphylococcus aureus
• 細胞内の位置: Cytoplasm (By similarity): Q6GJ88
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52142.89

構造登録者

Wang, H.C.,Ko, T.P.,Wang, A.H.J. (登録日: 2013-06-18, 公開日: 2014-02-05, 最終更新日: 2024-10-30)

主引用文献

Wang, H.C.,Hsu, K.C.,Yang, J.M.,Wu, M.L.,Ko, T.P.,Lin, S.R.,Wang, A.H.J.
Staphylococcus aureus protein SAUGI acts as a uracil-DNA glycosylase inhibitor.
Nucleic Acids Res., 42:1354-1364, 2013

PubMed Abstract: DNA mimic proteins are unique factors that control the DNA binding activity of target proteins by directly occupying their DNA binding sites. The extremely divergent amino acid sequences of the DNA mimics make these proteins hard to predict, and although they are likely to be ubiquitous, to date, only a few have been reported and functionally analyzed. Here we used a bioinformatic approach to look for potential DNA mimic proteins among previously reported protein structures. From ∼14 candidates, we selected the Staphylococcus conserved hypothetical protein SSP0047, and used proteomic and structural approaches to show that it is a novel DNA mimic protein. In Staphylococcus aureus, we found that this protein acts as a uracil-DNA glycosylase inhibitor, and therefore named it S. aureus uracil-DNA glycosylase inhibitor (SAUGI). We also determined and analyzed the complex structure of SAUGI and S. aureus uracil-DNA glycosylase (SAUDG). Subsequent BIAcore studies further showed that SAUGI has a high binding affinity to both S. aureus and human UDG. The two uracil-DNA glycosylase inhibitors (UGI and p56) previously known to science were both found in Bacillus phages, and this is the first report of a bacterial DNA mimic that may regulate SAUDG's functional roles in DNA repair and host defense.

PubMed: 24150946
DOI: 10.1093/nar/gkt964

実験手法

X-RAY DIFFRACTION (1.48 Å)