3WCT

The structure of a deoxygenated 400 kda hemoglobin provides a more accurate description of the cooperative mechanism of giant hemoglobins: Oxygenated form

3WCT の概要

• エントリーDOI: 10.2210/pdb3wct/pdb
• 関連するPDBエントリー: 3WCU 3WCV 3WCW
• 分子名称: A1 globin chain of giant V2 hemoglobin, A2 globin chain of giant V2 hemoglobin, B2 globin chain of giant V2 hemoglobin, ... (9 entities in total)
• 機能のキーワード: globin fold, oxygen transport, oxygen binding, blood
• 由来する生物種: Lamellibrachia satsuma (Hydrothermal vent tubeworm); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 137519.54

構造登録者

Numoto, N.,Nakagawa, T.,Ohara, R.,Hasegawa, T.,Kita, A.,Yoshida, T.,Maruyama, T.,Imai, K.,Fukumori, Y.,Miki, K. (登録日: 2013-06-01, 公開日: 2014-06-04, 最終更新日: 2024-10-30)

主引用文献

Numoto, N.,Nakagawa, T.,Ohara, R.,Hasegawa, T.,Kita, A.,Yoshida, T.,Maruyama, T.,Imai, K.,Fukumori, Y.,Miki, K.
The structure of a deoxygenated 400 kDa haemoglobin reveals ternary- and quaternary-structural changes of giant haemoglobins
Acta Crystallogr.,Sect.D, 70:1823-1831, 2014

PubMed Abstract: The quaternary structures of invertebrate haemoglobins (Hbs) are quite different from those of vertebrate Hbs. The extracellular giant Hbs of molecular masses of about 400 and 3600 kDa are composed of a dome-shaped dodecameric subassembly which consists of four individual globin subunits. Several crystal structures of 400 kDa Hbs from annelids have been reported, including structures in oxygenated and partially unliganded states, but the structure of the fully deoxygenated state has not been reported. In the present study, crystal structures of V2Hb from the tube worm Lamellibrachia satsuma have been determined in both the fully oxygenated and deoxygenated states. A glycosylation site and novel metal-binding sites for divalent cations were clearly observed with no intersubunit interactions in V2Hb. A comparison of the oxygenated and the deoxygenated forms of V2Hb reveals that the ternary- and quaternary-structural changes occur in a manner that maintains the molecular D3 symmetry. These structures suggest that the mechanisms of quaternary-structural changes between the oxy and deoxy states for the giant Hbs are identical across species.

PubMed: 25004960
DOI: 10.1107/S1399004714008475

実験手法

X-RAY DIFFRACTION (2.4 Å)