3WC7
Carboxypeptidase B in complex with EF6265
Summary for 3WC7
| Entry DOI | 10.2210/pdb3wc7/pdb |
| Related | 3WAB 3WC5 3WC6 |
| Descriptor | Carboxypeptidase B, ZINC ION, (2S)-7-amino-2-{[(R)-hydroxy{(1R)-2-methyl-1-[(3-phenylpropanoyl)amino]propyl}phosphoryl]methyl}heptanoic acid, ... (4 entities in total) |
| Functional Keywords | cpb inhibitor, ef6265, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor |
| Biological source | Sus scrofa (Pig) |
| Cellular location | Secreted: P09955 |
| Total number of polymer chains | 1 |
| Total formula weight | 35297.16 |
| Authors | Yoshimoto, N.,Itoh, T.,Inaba, Y.,Yamamoto, K. (deposition date: 2013-05-24, release date: 2013-10-02, Last modification date: 2024-10-30) |
| Primary citation | Yoshimoto, N.,Itoh, T.,Inaba, Y.,Ishii, H.,Yamamoto, K. Structural basis for inhibition of carboxypeptidase B by selenium-containing inhibitor: selenium coordinates to zinc in enzyme. J.Med.Chem., 56:7527-7535, 2013 Cited by PubMed Abstract: Activated thrombin-activatable fibrinolysis inhibitor (TAFIa) is a zinc-containing carboxypeptidase and significantly inhibits fibrinolysis. TAFIa inhibitors are thus expected to act as profibrinolytic agents. We recently reported the design and synthesis of selenium-containing inhibitors of TAFIa and their inhibitory activity. Here we report the crystal structures of potent selenium-, sulfur-, and phosphinic acid-containing inhibitors bound to porcine pancreatic carboxypeptidase B (ppCPB). ppCPB is a TAFIa homologue and is surrogate TAFIa for crystallographic analysis. Crystal structures of ppCPB complexed with selenium compound 1a, its sulfur analogue 2, and phosphinic acid derivative EF6265 were determined at 1.70, 2.15, and 1.90 Å resolution, respectively. Each inhibitor binds to the active site of ppCPB in a similar manner to that observed for previously reported inhibitors. Thus, in complexes, selenium, sulfur, and phosphinic acid oxygen coordinate to zinc in ppCPB. This is the first observation and report of selenium coordinating to zinc in CPB. PubMed: 24010887DOI: 10.1021/jm400816v PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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