3WBM

Crystal Structure of protein-RNA complex

3WBM の概要

• エントリーDOI: 10.2210/pdb3wbm/pdb
• 分子名称: DNA/RNA-binding protein Alba 1, RNA (25-MER) (3 entities in total)
• 機能のキーワード: protein-rna complex, dna/rna binding, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Sulfolobus shibatae; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 58305.31

構造登録者

Ding, J.,Wang, D.C. (登録日: 2013-05-20, 公開日: 2013-12-11, 最終更新日: 2023-11-08)

主引用文献

Guo, L.,Ding, J.,Guo, R.,Hou, Y.,Wang, D.C.,Huang, L.
Biochemical and structural insights into RNA binding by Ssh10b, a member of the highly conserved Sac10b protein family in Archaea.
J.Biol.Chem., 289:1478-1490, 2014

PubMed Abstract: Proteins of the Sac10b family are highly conserved in Archaea. Ssh10b, a member of the Sac10b family from the hyperthermophilic crenarchaeon Sulfolobus shibatae, binds to RNA in vivo. Here we show that binding by Ssh10b destabilizes RNA secondary structure. Structural analysis of Ssh10b in complex with a 25-bp RNA duplex containing local distortions reveals that Ssh10b binds the two RNA strands symmetrically as a tetramer with each dimer bound asymmetrically to a single RNA strand. Amino acid residues involved in double-stranded RNA binding are similar, but non-identical, to those in dsDNA binding. The dimer-dimer interaction mediated by the intermolecular β-sheet appears to facilitate the destabilization of base pairing in the secondary structure of RNA. Our results suggest that proteins of the Sac10b family may play important roles in RNA transactions requiring destabilization of RNA secondary structure in Sulfolobus.

PubMed: 24307170
DOI: 10.1074/jbc.M113.521351

実験手法

X-RAY DIFFRACTION (2 Å)