3WAM

Crystal structure of human LC3C_8-125

3WAM の概要

• エントリーDOI: 10.2210/pdb3wam/pdb
• 関連するPDBエントリー: 3VTU 3VTV 3VTW 3WAL 3WAN 3WAO 3WAP
• 分子名称: Microtubule-associated proteins 1A/1B light chain 3C, CITRIC ACID (3 entities in total)
• 機能のキーワード: ubiquitin-like fold, autophagy, protein transport
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, cytoskeleton: Q9BXW4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14144.31

構造登録者

Suzuki, H.,Tabata, K.,Morita, E.,Kawasaki, M.,Kato, R.,Dobson, R.C.J.,Yoshimori, T.,Wakatsuki, S. (登録日: 2013-05-06, 公開日: 2013-12-25, 最終更新日: 2023-11-08)

主引用文献

Suzuki, H.,Tabata, K.,Morita, E.,Kawasaki, M.,Kato, R.,Dobson, R.C.,Yoshimori, T.,Wakatsuki, S.
Structural basis of the autophagy-related LC3/Atg13 LIR complex: recognition and interaction mechanism.
Structure, 22:47-58, 2014

PubMed Abstract: Autophagy is a bulk degradation pathway that removes cytosolic materials to maintain cellular homeostasis. The autophagy-related gene 13 (Atg13) and microtubule associate protein 1 light chain 3 (LC3) proteins are required for autophagosome formation. We demonstrate that each of the human LC3 isoforms (LC3A, LC3B, and LC3C) interacts with Atg13 via the LC3 interacting region (LIR) of Atg13. Using X-ray crystallography, we solved the macromolecular structures of LC3A and LC3C, along with the complex structures of the LC3 isoforms with the Atg13 LIR. Together, our structural and binding analyses reveal that the side-chain of Lys49 of LC3 acts as a gatekeeper to regulate binding of the LIR. We verified this observation by mutation of Lys49 in LC3A, which significantly reduces LC3A positive puncta formation in cultured cells. Our results suggest that specific affinity of the LC3 isoforms to the Atg13 LIR is required for proper autophagosome formation.

PubMed: 24290141
DOI: 10.1016/j.str.2013.09.023

実験手法

X-RAY DIFFRACTION (1.75 Å)