3WAE
X-ray structure of Fe(III)-bicarbonates-ttfbpa, a ferric ion-binding protein from thermus thermophilus HB8
Summary for 3WAE
| Entry DOI | 10.2210/pdb3wae/pdb |
| Related | 3WAF |
| Descriptor | IRON ABC TRANSPORTER, PERIPLASMIC IRON-BINDING PROTEIN, FE (III) ION, BICARBONATE ION, ... (4 entities in total) |
| Functional Keywords | a ferric binding protein, ferric ion, metal binding protein |
| Biological source | Thermus thermophilus |
| Total number of polymer chains | 2 |
| Total formula weight | 72743.45 |
| Authors | Wang, S.,Ogata, M.,Horita, S.,Ohtsuka, J.,Nagata, K.,Tanokura, M. (deposition date: 2013-05-02, release date: 2014-05-21, Last modification date: 2024-03-20) |
| Primary citation | Wang, S.,Ogata, M.,Horita, S.,Ohtsuka, J.,Nagata, K.,Tanokura, M. A novel mode of ferric ion coordination by the periplasmic ferric ion-binding subunit FbpA of an ABC-type iron transporter from Thermus thermophilus HB8. Acta Crystallogr.,Sect.D, 70:196-202, 2014 Cited by PubMed Abstract: Crystal structures of FbpA, the periplasmic ferric ion-binding protein of an iron-uptake ABC transporter, from Thermus thermophilus HB8 (TtFbpA) have been solved in apo and ferric ion-bound forms at 1.8 and 1.7 Å resolution, respectively. The latter crystal structure shows that the bound ferric ion forms a novel six-coordinated complex with three tyrosine side chains, two bicarbonates and a water molecule in the metal-binding site. The results of gel-filtration chromatography and dynamic light scattering show that TtFbpA exists as a monomer in solution regardless of ferric ion binding and that TtFbpA adopts a more compact conformation in the ferric ion-bound state than in the apo state in solution. PubMed: 24419392DOI: 10.1107/S1399004713026333 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.7 Å) |
Structure validation
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