3WAE

X-ray structure of Fe(III)-bicarbonates-ttfbpa, a ferric ion-binding protein from thermus thermophilus HB8

3WAE の概要

• エントリーDOI: 10.2210/pdb3wae/pdb
• 関連するPDBエントリー: 3WAF
• 分子名称: IRON ABC TRANSPORTER, PERIPLASMIC IRON-BINDING PROTEIN, FE (III) ION, BICARBONATE ION, ... (4 entities in total)
• 機能のキーワード: a ferric binding protein, ferric ion, metal binding protein
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72743.45

構造登録者

Wang, S.,Ogata, M.,Horita, S.,Ohtsuka, J.,Nagata, K.,Tanokura, M. (登録日: 2013-05-02, 公開日: 2014-05-21, 最終更新日: 2024-03-20)

主引用文献

Wang, S.,Ogata, M.,Horita, S.,Ohtsuka, J.,Nagata, K.,Tanokura, M.
A novel mode of ferric ion coordination by the periplasmic ferric ion-binding subunit FbpA of an ABC-type iron transporter from Thermus thermophilus HB8.
Acta Crystallogr.,Sect.D, 70:196-202, 2014

PubMed Abstract: Crystal structures of FbpA, the periplasmic ferric ion-binding protein of an iron-uptake ABC transporter, from Thermus thermophilus HB8 (TtFbpA) have been solved in apo and ferric ion-bound forms at 1.8 and 1.7 Å resolution, respectively. The latter crystal structure shows that the bound ferric ion forms a novel six-coordinated complex with three tyrosine side chains, two bicarbonates and a water molecule in the metal-binding site. The results of gel-filtration chromatography and dynamic light scattering show that TtFbpA exists as a monomer in solution regardless of ferric ion binding and that TtFbpA adopts a more compact conformation in the ferric ion-bound state than in the apo state in solution.

PubMed: 24419392
DOI: 10.1107/S1399004713026333

実験手法

X-RAY DIFFRACTION (1.7 Å)