3WA6

Crystal structure of tannase from Lactobacillus plantarum in the orthorhombic crystal

3WA6 の概要

• エントリーDOI: 10.2210/pdb3wa6/pdb
• 分子名称: tannase, SULFATE ION (3 entities in total)
• 機能のキーワード: alpha/beta-hydrolase, hydrolase
• 由来する生物種: Lactobacillus plantarum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52028.04

構造登録者

Matoba, Y.,Tanaka, N.,Sugiyama, M. (登録日: 2013-04-27, 公開日: 2013-07-24, 最終更新日: 2024-03-20)

主引用文献

Matoba, Y.,Tanaka, N.,Noda, M.,Higashikawa, F.,Kumagai, T.,Sugiyama, M.
Crystallographic and mutational analyses of tannase from Lactobacillus plantarum
Proteins, 81:2052-2058, 2013

PubMed Abstract: Tannin acylhydrolase (EC 3.1.1.20) referred commonly as tannase catalyzes the hydrolysis of the galloyl ester bond of tannins to release gallic acid. Although the enzyme is useful for various industries, the tertiary structure is not yet determined. In this study, we determined the crystal structure of tannase produced by Lactobacillus plantarum. The tannase structure belongs to a member of α/β-hydrolase superfamily with an additional "lid" domain. A glycerol molecule derived from cryoprotectant solution was accommodated into the tannase active site. The binding manner of glycerol to tannase seems to be similar to that of the galloyl moiety in the substrate.

PubMed: 23836494
DOI: 10.1002/prot.24355

実験手法

X-RAY DIFFRACTION (1.8 Å)