3W9K

Crystal structure of thermoacidophile-specific protein STK_08120 complexed with myristic acid

3W9K の概要

• エントリーDOI: 10.2210/pdb3w9k/pdb
• 関連するPDBエントリー: 2EJX
• 分子名称: FATTY ACID-BINDING PROTEIN, MYRISTIC ACID (3 entities in total)
• 機能のキーワード: helix-grip fold, fatty acid binding, lipid binding protein
• 由来する生物種: Sulfolobus tokodaii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16243.94

構造登録者

Miyakawa, T.,Sawano, Y.,Miyazono, K.,Miyauchi, Y.,Hatano, K.,Tanokura, M. (登録日: 2013-04-05, 公開日: 2013-07-17, 最終更新日: 2023-11-08)

主引用文献

Miyakawa, T.,Sawano, Y.,Miyazono, K.,Miyauchi, Y.,Hatano, K.,Tanokura, M.
A thermoacidophile-specific protein family, DUF3211, functions as a fatty acid carrier with novel binding mode.
J.Bacteriol., 195:4005-4012, 2013

PubMed Abstract: STK_08120 is a member of the thermoacidophile-specific DUF3211 protein family from Sulfolobus tokodaii strain 7. Its molecular function remains obscure, and sequence similarities for obtaining functional remarks are not available. In this study, the crystal structure of STK_08120 was determined at 1.79-Å resolution to predict its probable function using structure similarity searches. The structure adopts an α/β structure of a helix-grip fold, which is found in the START domain proteins with cavities for hydrophobic substrates or ligands. The detailed structural features implied that fatty acids are the primary ligand candidates for STK_08120, and binding assays revealed that the protein bound long-chain saturated fatty acids (>C14) and their trans-unsaturated types with an affinity equal to that for major fatty acid binding proteins in mammals and plants. Moreover, the structure of an STK_08120-myristic acid complex revealed a unique binding mode among fatty acid binding proteins. These results suggest that the thermoacidophile-specific protein family DUF3211 functions as a fatty acid carrier with a novel binding mode.

PubMed: 23836863
DOI: 10.1128/JB.00432-13

実験手法

X-RAY DIFFRACTION (1.8 Å)