3W98

Crystal Structure of Human Nucleosome Core Particle lacking H3.1 N-terminal region

3W98 の概要

• エントリーDOI: 10.2210/pdb3w98/pdb
• 関連するPDBエントリー: 3AFA 3W96 3W97 3W99
• 分子名称: Histone H3.1, Histone H4, Histone H2A type 1-B/E, ... (6 entities in total)
• 機能のキーワード: protein-dna complex, histone-fold, nucleosome, structural protein-dna complex, structural protein/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P68431 P62805 P04908 P06899
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 196480.72

構造登録者

Iwasaki, W.,Miya, Y.,Horikoshi, N.,Osakabe, A.,Tachiwana, H.,Shibata, T.,Kagawa, W.,Kurumizaka, H. (登録日: 2013-04-01, 公開日: 2013-08-28, 最終更新日: 2023-11-08)

主引用文献

Iwasaki, W.,Miya, Y.,Horikoshi, N.,Osakabe, A.,Taguchi, H.,Tachiwana, H.,Shibata, T.,Kagawa, W.,Kurumizaka, H.
Contribution of histone N-terminal tails to the structure and stability of nucleosomes
FEBS Open Bio, 3:363-369, 2013

PubMed Abstract: Histones are the protein components of the nucleosome, which forms the basic architecture of eukaryotic chromatin. Histones H2A, H2B, H3, and H4 are composed of two common regions, the "histone fold" and the "histone tail". Many efforts have been focused on the mechanisms by which the post-translational modifications of histone tails regulate the higher-order chromatin architecture. On the other hand, previous biochemical studies have suggested that histone tails also affect the structure and stability of the nucleosome core particle itself. However, the precise contributions of each histone tail are unclear. In the present study, we determined the crystal structures of four mutant nucleosomes, in which one of the four histones, H2A, H2B, H3, or H4, lacked the N-terminal tail. We found that the deletion of the H2B or H3 N-terminal tail affected histone-DNA interactions and substantially decreased nucleosome stability. These findings provide important information for understanding the complex roles of histone tails in regulating chromatin structure.

PubMed: 24251097
DOI: 10.1016/j.fob.2013.08.007

実験手法

X-RAY DIFFRACTION (3.42 Å)