3W8K

Crystal structure of class C beta-lactamase Mox-1

3W8K の概要

• エントリーDOI: 10.2210/pdb3w8k/pdb
• 分子名称: Beta-lactamase, ZINC ION, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: beta-lactamase, antibiotic resistance, hydrolase
• 由来する生物種: Klebsiella pneumoniae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41684.65

構造登録者

Shimizu-ibuka, A.,Oguri, T.,Furuyama, T.,Ishii, Y. (登録日: 2013-03-15, 公開日: 2014-04-23, 最終更新日: 2024-10-30)

主引用文献

Oguri, T.,Furuyama, T.,Okuno, T.,Ishii, Y.,Tateda, K.,Bonomo, R.A.,Shimizu-Ibuka, A.
Crystal structure of Mox-1, a unique plasmid-mediated class C beta-lactamase with hydrolytic activity towards moxalactam
Antimicrob.Agents Chemother., 58:3914-3920, 2014

PubMed Abstract: Mox-1 is a unique plasmid-mediated class C β-lactamase that hydrolyzes penicillins, cephalothin, and the expanded-spectrum cephalosporins cefepime and moxalactam. In order to understand the unique substrate profile of this enzyme, we determined the X-ray crystallographic structure of Mox-1 β-lactamase at a 1.5-Å resolution. The overall structure of Mox-1 β-lactamase resembles that of other AmpC enzymes, with some notable exceptions. First, comparison with other enzymes whose structures have been solved reveals significant differences in the composition of amino acids that make up the hydrogen-bonding network and the position of structural elements in the substrate-binding cavity. Second, the main-chain electron density is not observed in two regions, one containing amino acid residues 214 to 216 positioned in the Ω loop and the other in the N terminus of the B3 β-strand corresponding to amino acid residues 303 to 306. The last two observations suggest that there is significant structural flexibility of these regions, a property which may impact the recognition and binding of substrates in Mox-1. These important differences allow us to propose that the binding of moxalactam in Mox-1 is facilitated by the avoidance of steric clashes, indicating that a substrate-induced conformational change underlies the basis of the hydrolytic profile of Mox-1 β-lactamase.

PubMed: 24777102
DOI: 10.1128/AAC.02363-13

実験手法

X-RAY DIFFRACTION (1.5 Å)