3W6R

Crystal structure of the GAP domain of human MgcRacGAP

3W6R の概要

• エントリーDOI: 10.2210/pdb3w6r/pdb
• 分子名称: Rac GTPase-activating protein 1 (2 entities in total)
• 機能のキーワード: gtpase activating, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q9H0H5
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22788.62

構造登録者

Matsuura, A.,Lee, H.H. (登録日: 2013-02-21, 公開日: 2014-02-26, 最終更新日: 2023-11-08)

主引用文献

Matsuura, A.,Lee, H.H.
Crystal structure of GTPase-activating domain from human MgcRacGAP.
Biochem.Biophys.Res.Commun., 435:367-372, 2013

PubMed Abstract: Cytokinesis in animal cells relies on a centralspindlin complex consisting of male germ cell RacGap (MgcRacGAP) and mitotic kinesin-like protein 1 (MKLP1). Rho GTPases act as molecular switches to regulate the actin cytoskeleton for cytokinesis, of which Rac1 is regulated by MgcRacGAP. In this study, we determined the crystal structure of the GTPase-activating protein (GAP) domain of MgcRacGAP at a resolution of 1.9Å. The conformation of Arg385, which is a key residue for GAP activity, was found to be different from that of previously reported GAP proteins, and MgcRacGAP (residues 348-546) was found to exist as a monomer in solution, according to Stokes radii. We also measured the GAP activity of MgcRacGAP mutants for Rac1.

PubMed: 23665020
DOI: 10.1016/j.bbrc.2013.04.094

実験手法

X-RAY DIFFRACTION (1.9 Å)