3W6K

Crystal structure of dimer of ScpB N-terminal domain complexed with ScpA peptide

3W6K の概要

• エントリーDOI: 10.2210/pdb3w6k/pdb
• 関連するPDBエントリー: 3W6J
• 分子名称: ScpA, ScpB (3 entities in total)
• 機能のキーワード: regulatory subcomplex, smc, winged hth, cell cycle
• 由来する生物種: Geobacillus stearothermophilus; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 43037.55

構造登録者

Kamada, K.,Hirano, T. (登録日: 2013-02-15, 公開日: 2013-04-24, 最終更新日: 2024-04-03)

主引用文献

Kamada, K.,Miyata, M.,Hirano, T.
Molecular basis of SMC ATPase activation: role of internal structural changes of the regulatory subcomplex ScpAB
Structure, 21:581-594, 2013

PubMed Abstract: In many bacteria, a homodimer of structural-maintenance-of-chromosomes proteins associates with two regulatory subunits (known as ScpA and ScpB), assembling a protein complex that plays a crucial role in chromosome organization and segregation. It remains poorly understood, however, how this complex might work at the mechanistic level. Here, we report crystal structures of the ScpAB core complex that display a highly unusual structure in which the central segment of ScpA winds around an asymmetrically oriented ScpB dimer. The two C-terminal domains of the ScpB dimer primarily interact with different regions of ScpA with different affinities. Moreover, flexible interdomain regions of ScpB contribute to a dynamic folding process of the ScpAB subcomplex. Together with other genetic and biochemical assays, we provide evidence that internal structural changes of the ScpAB subcomplex are tightly coupled with activation of the structural-maintenance-of-chromosomes ATPase.

PubMed: 23541893
DOI: 10.1016/j.str.2013.02.016

実験手法

X-RAY DIFFRACTION (2.374 Å)