3W6D

Crystal structure of catalytic domain of chitinase from Ralstonia sp. A-471 (E141Q) in complex with tetrasaccharide

3W6D の概要

• エントリーDOI: 10.2210/pdb3w6d/pdb
• 関連するPDBエントリー: 3W6B 3W6C 3W6E 3W6F
• 関連するBIRD辞書のPRD_ID: PRD_900017
• 分子名称: Lysozyme-like chitinolytic enzyme, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: gh family 23, enzyme, glycoside hydrolase, chitinase, hydrolase
• 由来する生物種: Ralstonia
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 84461.81

構造登録者

Arimori, T.,Kawamoto, N.,Okazaki, N.,Nakazawa, M.,Miyatake, K.,Fukamizo, T.,Ueda, M.,Tamada, T. (登録日: 2013-02-14, 公開日: 2013-05-15, 最終更新日: 2023-11-08)

主引用文献

Arimori, T.,Kawamoto, N.,Shinya, S.,Okazaki, N.,Nakazawa, M.,Miyatake, K.,Fukamizo, T.,Ueda, M.,Tamada, T.
Crystal Structures of the Catalytic Domain of a Novel Glycohydrolase Family 23 Chitinase from Ralstonia sp. A-471 Reveals a Unique Arrangement of the Catalytic Residues for Inverting Chitin Hydrolysis
J.Biol.Chem., 288:18696-18706, 2013

PubMed Abstract: Chitinase C from Ralstonia sp. A-471 (Ra-ChiC) has a catalytic domain sequence similar to goose-type (G-type) lysozymes and, unlike other chitinases, belongs to glycohydrolase (GH) family 23. Using NMR spectroscopy, however, Ra-ChiC was found to interact only with the chitin dimer but not with the peptidoglycan fragment. Here we report the crystal structures of wild-type, E141Q, and E162Q of the catalytic domain of Ra-ChiC with or without chitin oligosaccharides. Ra-ChiC has a substrate-binding site including a tunnel-shaped cavity, which determines the substrate specificity. Mutation analyses based on this structural information indicated that a highly conserved Glu-141 acts as a catalytic acid, and that Asp-226 located at the roof of the tunnel activates a water molecule as a catalytic base. The unique arrangement of the catalytic residues makes a clear contrast to the other GH23 members and also to inverting GH19 chitinases.

PubMed: 23658014
DOI: 10.1074/jbc.M113.462135

実験手法

X-RAY DIFFRACTION (2.15 Å)