3W61

MamM-CTD H285A

Summary for 3W61

• Entry DOI: 10.2210/pdb3w61/pdb
• Related: 3W5X 3W5Y 3W5Z 3W60 3W62 3W63 3W64 3W65 3W66 3W8P
• Descriptor: Magnetosome protein MamM, SULFATE ION (3 entities in total)
• Functional Keywords: cation diffusion facilitator (cdf), divalent cation transport, metal ion transport, metal transport
• Biological source: Magnetospirillum gryphiswaldense
• Total number of polymer chains: 1
• Total formula weight: 12111.57

Authors

Zeytuni, N.,Davidov, G.,Zarivach, R. (deposition date: 2013-02-10, release date: 2014-04-16, Last modification date: 2024-10-30)

Primary citation

Zeytuni, N.,Uebe, R.,Maes, M.,Davidov, G.,Baram, M.,Raschdorf, O.,Nadav-Tsubery, M.,Kolusheva, S.,Bitton, R.,Goobes, G.,Friedler, A.,Miller, Y.,Schuler, D.,Zarivach, R.
Cation diffusion facilitators transport initiation and regulation is mediated by cation induced conformational changes of the cytoplasmic domain
Plos One, 9:e92141-e92141, 2014

PubMed Abstract: Cation diffusion facilitators (CDF) are part of a highly conserved protein family that maintains cellular divalent cation homeostasis in all domains of life. CDF's were shown to be involved in several human diseases, such as Type-II diabetes and neurodegenerative diseases. In this work, we employed a multi-disciplinary approach to study the activation mechanism of the CDF protein family. For this we used MamM, one of the main ion transporters of magnetosomes--bacterial organelles that enable magnetotactic bacteria to orientate along geomagnetic fields. Our results reveal that the cytosolic domain of MamM forms a stable dimer that undergoes distinct conformational changes upon divalent cation binding. MamM conformational change is associated with three metal binding sites that were identified and characterized. Altogether, our results provide a novel auto-regulation mode of action model in which the cytosolic domain's conformational changes upon ligand binding allows the priming of the CDF into its transport mode.

PubMed: 24658343
DOI: 10.1371/journal.pone.0092141

Experimental method

X-RAY DIFFRACTION (1.59 Å)