3W0E

Structure of elastase inhibitor AFUEI (crystal form II)

3W0E の概要

• エントリーDOI: 10.2210/pdb3w0e/pdb
• 関連するPDBエントリー: 3W0D
• 分子名称: Elastase inhibitor AFUEI (2 entities in total)
• 機能のキーワード: elastase inhibitor, secreted protein, hydrolase inhibitor
• 由来する生物種: Aspergillus fumigatus Af293
• 細胞内の位置: Secreted: Q4WZ11
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15062.90

構造登録者

Imada, K.,Sakuma, M.,Okumura, Y.,Ogawa, K.,Nikai, T.,Homma, M. (登録日: 2012-10-29, 公開日: 2013-05-15, 最終更新日: 2024-10-30)

主引用文献

Sakuma, M.,Imada, K.,Okumura, Y.,Uchiya, K.,Yamashita, N.,Ogawa, K.,Hijikata, A.,Shirai, T.,Homma, M.,Nikai, T.
X-ray Structure Analysis and Characterization of AFUEI, an Elastase Inhibitor from Aspergillus fumigatus
J.Biol.Chem., 288:17451-17459, 2013

PubMed Abstract: Elastase from Aspergillus sp. is an important factor for aspergillosis. AFUEI is an inhibitor of the elastase derived from Aspergillus fumigatus. AFUEI is a member of the I78 inhibitor family and has a high inhibitory activity against elastases of Aspergillus fumigatus and Aspergillus flavus, human neutrophil elastase and bovine chymotrypsin, but does not inhibit bovine trypsin. Here we report the crystal structure of AFUEI in two crystal forms. AFUEI is a wedge-shaped protein composed of an extended loop and a scaffold protein core. The structure of AFUEI shows remarkable similarity to serine protease inhibitors of the potato inhibitor I family, although they are classified into different inhibitor families. A structural comparison with the potato I family inhibitors suggests that the extended loop of AFUEI corresponds to the binding loop of the potato inhibitor I family, and AFUEI inhibits its cognate proteases through the same mechanism as the potato I family inhibitors.

PubMed: 23640894
DOI: 10.1074/jbc.M112.433987

実験手法

X-RAY DIFFRACTION (1.8 Å)