3VV3

Crystal structure of deseasin MCP-01 from Pseudoalteromonas sp. SM9913

3VV3 の概要

• エントリーDOI: 10.2210/pdb3vv3/pdb
• 分子名称: Deseasin MCP-01, CALCIUM ION (3 entities in total)
• 機能のキーワード: alpha/beta, protease, hydrolase
• 由来する生物種: Pseudoalteromonas sp.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68049.52

構造登録者

Zhao, G.Y.,Gao, X.,Chen, X.L.,Zhang, Y.Z. (登録日: 2012-07-14, 公開日: 2013-08-07, 最終更新日: 2023-11-08)

主引用文献

Ran, L.Y.,Su, H.N.,Zhao, G.Y.,Gao, X.,Zhou, M.Y.,Wang, P.,Zhao, H.L.,Xie, B.B.,Zhang, X.Y.,Chen, X.L.,Zhou, B.C.,Zhang, Y.Z.
Structural and mechanistic insights into collagen degradation by a bacterial collagenolytic serine protease in the subtilisin family.
Mol. Microbiol., 90:997-1010, 2013

PubMed Abstract: A number of proteases in the subtilisin family derived from environmental or pathogenic microorganisms have been reported to be collagenolytic serine proteases. However, their collagen degradation mechanisms remain unclear. Here, the degradation mechanism of type I collagen fibres by the S8 collagenolytic protease MCP-01, from Pseudoalteromonas sp. SM9913, was studied. Atomic force microscopy observation and biochemical analysis confirmed that MCP-01 progressively released single fibrils from collagen fibres and released collagen monomers from fibrils mainly by hydrolysing proteoglycans and telopeptides in the collagen fibres. Structural and mutational analyses indicated that an enlarged substrate-binding pocket, mainly composed of loops 7, 9 and 11, is necessary for collagen recognition and that the acidic and aromatic residues on these loops form a negatively charged, hydrophobic environment for collagen binding. MCP-01 displayed a non-strict preference for peptide bonds with Pro or basic residues at the P1 site and/or Gly at the P1' site in collagen. His211 is a key residue for the P1-basic-residue preference of MCP-01. Our study gives structural and mechanistic insights into collagen degradation of the S8 collagenolytic protease, which is helpful in developing therapeutics for diseases with S8 collagenolytic proteases as pathogenic factors and in studying environmental organic nitrogen degradation mechanisms.

PubMed: 24112706
DOI: 10.1111/mmi.12412

実験手法

X-RAY DIFFRACTION (1.76 Å)