3VUF

Crystal Structure of Rice Granule bound Starch Synthase I Catalytic Domain in Complex with ADP

3VUF の概要

• エントリーDOI: 10.2210/pdb3vuf/pdb
• 関連するPDBエントリー: 3VUE
• 分子名称: Granule-bound starch synthase 1, chloroplastic/amyloplastic, ADENOSINE-5'-DIPHOSPHATE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: rossmann fold, glycosyltransferase, transferase
• 由来する生物種: Oryza sativa Japonica Group (Japanese rice)
• 細胞内の位置: Plastid, chloroplast: Q0DEV5
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 60069.39

構造登録者

Momma, M.,Fujimoto, Z. (登録日: 2012-06-28, 公開日: 2012-09-05, 最終更新日: 2023-11-08)

主引用文献

Momma, M.,Fujimoto, Z.
Interdomain Disulfide Bridge in the Rice Granule Bound Starch Synthase I Catalytic Domain as Elucidated by X-Ray Structure Analysis
Biosci.Biotechnol.Biochem., 76:1591-1595, 2012

PubMed Abstract: The catalytic domain of rice (Oryza sativa japonica) granule bound starch synthase I (OsGBSSI-CD) was overexpressed and the three-dimensional structures of the ligand-free and ADP-bound forms were determined. The structures were similar to those reported for bacterial and archaeal glycogen synthases, which belong to glycosyltransferase family 5. They had Rossmann fold N- and C-domains connected by canonical two-hinge peptides, and an interdomain disulfide bond that appears to be conserved in the Poaceae plant family. The presence of three covalent linkages might explain why both OsGBSSI-CD structures adopted only the closed domain arrangement.

PubMed: 22878205
DOI: 10.1271/bbb.120305

実験手法

X-RAY DIFFRACTION (3 Å)