3VO1

Crystal structure of Zea mays leaf ferredoxin-NADP+ reductase II

Summary for 3VO1

• Entry DOI: 10.2210/pdb3vo1/pdb
• Related: 1GAW 3VO2
• Descriptor: Ferredoxin, FLAVIN-ADENINE DINUCLEOTIDE (3 entities in total)
• Functional Keywords: rossmann fold, oxidoreductase, fad binding
• Biological source: Zea mays (maize)
• Total number of polymer chains: 2
• Total formula weight: 72808.80

Authors

Muraki, N.,Hase, T.,Kurisu, G. (deposition date: 2012-01-18, release date: 2012-12-05, Last modification date: 2023-11-08)

Primary citation

Twachtmann, M.,Altmann, B.,Muraki, N.,Voss, I.,Okutani, S.,Kurisu, G.,Hase, T.,Hanke, G.T.
N-terminal structure of maize ferredoxin:NADP+ reductase determines recruitment into different thylakoid membrane complexes
Plant Cell, 24:2979-2991, 2012

PubMed Abstract: To adapt to different light intensities, photosynthetic organisms manipulate the flow of electrons through several alternative pathways at the thylakoid membrane. The enzyme ferredoxin:NADP(+) reductase (FNR) has the potential to regulate this electron partitioning because it is integral to most of these electron cascades and can associate with several different membrane complexes. However, the factors controlling relative localization of FNR to different membrane complexes have not yet been established. Maize (Zea mays) contains three chloroplast FNR proteins with totally different membrane association, and we found that these proteins have variable distribution between cells conducting predominantly cyclic electron transport (bundle sheath) and linear electron transport (mesophyll). Here, the crystal structures of all three enzymes were solved, revealing major structural differences at the N-terminal domain and dimer interface. Expression in Arabidopsis thaliana of maize FNRs as chimeras and truncated proteins showed the N-terminal determines recruitment of FNR to different membrane complexes. In addition, the different maize FNR proteins localized to different thylakoid membrane complexes on expression in Arabidopsis, and analysis of chlorophyll fluorescence and photosystem I absorbance demonstrates the impact of FNR location on photosynthetic electron flow.

PubMed: 22805436
DOI: 10.1105/tpc.111.094532

Experimental method

X-RAY DIFFRACTION (2 Å)