3VMG

Reduced carbazole-bound complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase

3VMG の概要

• エントリーDOI: 10.2210/pdb3vmg/pdb
• 関連するPDBエントリー: 3VMH 3VMI
• 分子名称: Terminal oxygenase component of carbazole, Ferredoxin component of carbazole, FE (II) ION, ... (6 entities in total)
• 機能のキーワード: catalytic mechanism, electron transfer complex, rieske nonheme iron oxygenase system, terminal oxygenase, rieske-type ferredoxin, carbazole 1, 9a-dioxygenase, oxidoreductase
• 由来する生物種: Janthinobacterium; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 173633.26

構造登録者

Ashikawa, Y.,Nojiri, H. (登録日: 2011-12-12, 公開日: 2012-08-22, 最終更新日: 2023-11-08)

主引用文献

Ashikawa, Y.,Fujimoto, Z.,Usami, Y.,Inoue, K.,Noguchi, H.,Yamane, H.,Nojiri, H.
Structural insight into the substrate- and dioxygenbinding manner in the catalytic cycle of rieske nonheme iron oxygenase system, carbazole 1,9adioxygenase
Bmc Struct.Biol., 12:15-15, 2012

PubMed Abstract: Dihydroxylation of tandemly linked aromatic carbons in a cis-configuration, catalyzed by multicomponent oxygenase systems known as Rieske nonheme iron oxygenase systems (ROs), often constitute the initial step of aerobic degradation pathways for various aromatic compounds. Because such RO reactions inherently govern whether downstream degradation processes occur, novel oxygenation mechanisms involving oxygenase components of ROs (RO-Os) is of great interest. Despite substantial progress in structural and physicochemical analyses, no consensus exists on the chemical steps in the catalytic cycles of ROs. Thus, determining whether conformational changes at the active site of RO-O occur by substrate and/or oxygen binding is important. Carbazole 1,9a-dioxygenase (CARDO), a RO member consists of catalytic terminal oxygenase (CARDO-O), ferredoxin (CARDO-F), and ferredoxin reductase. We have succeeded in determining the crystal structures of oxidized CARDO-O, oxidized CARDO-F, and both oxidized and reduced forms of the CARDO-O: CARDO-F binary complex.

PubMed: 22727022
DOI: 10.1186/1472-6807-12-15

実験手法

X-RAY DIFFRACTION (1.95 Å)