3VKS

Assimilatory nitrite reductase (Nii3) - NO complex from tobbaco leaf

3VKS の概要

• エントリーDOI: 10.2210/pdb3vks/pdb
• 関連するPDBエントリー: 3B0G 3VKP 3VKQ 3VKR 3VKT
• 分子名称: Nitrite reductase, SIROHEME, IRON/SULFUR CLUSTER, ... (7 entities in total)
• 機能のキーワード: 3 alpha/beta domains, reductase, siroheme, fe4s4, nitric oxide, oxidoreductase
• 由来する生物種: Nicotiana tabacum (tobacco)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 67869.21

構造登録者

Nakano, S.,Takahashi, M.,Sakamoto, A.,Morikawa, H.,Katayanagi, K. (登録日: 2011-11-20, 公開日: 2012-04-25, 最終更新日: 2023-12-27)

主引用文献

Nakano, S.,Takahashi, M.,Sakamoto, A.,Morikawa, H.,Katayanagi, K.
The reductive reaction mechanism of tobacco nitrite reductase derived from a combination of crystal structures and ultraviolet-visible microspectroscopy
Proteins, 80:2035-2045, 2012

PubMed Abstract: Assimilatory nitrite reductase (aNiR) reduces nitrite to an ammonium ion and has siroheme and a [Fe(4)S(4)] cluster as prosthetic groups. A reaction mechanism for Nii3, an aNiR from tobacco, is proposed based on high resolution X-ray structures and UV-Vis (ultraviolet-visible) microspectroscopy of Nii3-ligand complexes. Analysis of UV-Vis spectral changes in Nii3 crystals with increasing X-ray exposure showed prosthetic group reductions. In Nii3-NO2(-) structures, X-ray irradiation enhanced the progress of the reduction reaction, and cleavage of the N-O bond was observed when X-ray doses were increased. Crystal structures of Nii3 with other bound ligands, such as Nii3-NO and Nii3-NH(2)OH, were also determined. Further, by combining information from these Nii3 ligand-bound structures, including that of Nii3-NO2(-), with UV-Vis microspectral data obtained using different X-ray doses, a reaction mechanism for aNiR was suggested. Cleavage of the two N-O bonds of nitrite was envisaged as a two-step process: first, the N-O bond close to Lys224 was cleaved, followed by cleavage of the N-O bond close to Arg109. X-ray structures also indicated that aNiR-catalyzed nitrite reduction proceeded without the need for conformation changes in active site residues. Geometrical changes in the ligand molecules and the placement of neighboring water molecules appeared to be important to the stability of the active site residue interactions (Arg109, Arg179, and Lys224) and the ligand molecule. These interactions may contribute to the efficiency of aNiR reduction reactions.

PubMed: 22499059
DOI: 10.1002/prot.24094

実験手法

X-RAY DIFFRACTION (1.4 Å)