3VHM

Crystal structure of NPC-biotin-avidin complex

3VHM の概要

• エントリーDOI: 10.2210/pdb3vhm/pdb
• 関連するPDBエントリー: 3VGW 3VHH 3VHI
• 分子名称: Avidin, 5-[(3aS,4R,6aR)-1-{[(1R)-1-(6-nitro-1,3-benzodioxol-5-yl)ethoxy]carbonyl}-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoic acid, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: beta barrel, biotin-binding protein
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Secreted: P02701
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 58252.84

構造登録者

Terai, T.,Maki, E.,Sugiyama, S.,Takahashi, Y.,Matsumura, H.,Mori, Y.,Nagano, T. (登録日: 2011-08-29, 公開日: 2011-12-28, 最終更新日: 2024-10-30)

主引用文献

Terai, T.,Maki, E.,Sugiyama, S.,Takahashi, Y.,Matsumura, H.,Mori, Y.,Nagano, T.
Rational development of caged-biotin protein-labeling agents and some applications in live cells
Chem.Biol., 18:1261-1272, 2011

PubMed Abstract: Biotin-(strept)avidin complex is widely used in biotechnology because of its extremely high binding constant, but there is no report describing spatiotemporally controlled formation of the complex in live cells. Here, based on X-ray crystal structure analysis and calorimetric data, we designed and synthesized photoreleasable biotins, which show greatly reduced affinity for (strept)avidin, but recover native affinity after UV irradiation. For application at the cell surface, we introduced an amine-reactive moiety into these "caged" biotin molecules. Specific fluorescence imaging of live cells that had been labeled with these agents and then UV-irradiated, was accomplished by addition of streptavidin conjugated with a fluorophore. We also demonstrated the applicability of these compounds for UV-irradiated-cell-specific drug delivery by using caged-biotin-labeled cells, a prodrug, and streptavidin conjugated with a prodrug-activating enzyme.

PubMed: 22035795
DOI: 10.1016/j.chembiol.2011.09.007

実験手法

X-RAY DIFFRACTION (2 Å)