3VG2

Iodide derivative of human LFABP

3VG2 の概要

• エントリーDOI: 10.2210/pdb3vg2/pdb
• 関連するPDBエントリー: 3B2H 3B2I 3B2J 3B2K 3B2L 3VG3 3VG4 3VG5 3VG6 3VG7
• 分子名称: Fatty acid-binding protein, liver, PALMITIC ACID, IODIDE ION, ... (4 entities in total)
• 機能のキーワード: lfabp, iodide, copper kalpha, palmitic acid, lipid binding protein
• 由来する生物種: Homo Sapiens
• 細胞内の位置: Cytoplasm: P07148
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15747.10

構造登録者

Sharma, A.,Yogavel, M.,Sharma, A. (登録日: 2011-08-03, 公開日: 2012-06-20, 最終更新日: 2024-03-20)

主引用文献

Sharma, A.,Yogavel, M.,Sharma, A.
Utility of anion and cation combinations for phasing of protein structures.
J.Struct.Funct.Genom., 13:135-143, 2012

PubMed Abstract: We report the use of anionic (I(-)), cationic (Ba(2+), Cd(2+)) and ionic mixtures (I(-) plus Ba(2+)) for derivatizing liver fatty acid binding protein (LFABP) crystals. Use of cationic and anionic salts in phasing experiments revealed distinct non-overlapping sites for these ions, suggesting exclusive binding regions on LFABP. Interestingly, cations of identical charge and valency (like Ba(2+) and Cd(2+)) bound to distinct pockets on the protein surface. Furthermore, a mixture of salts containing both I(-) and Ba(2+) was very useful in phasing experiments as these oppositely charged ions bound to different regions of LFABP. Our data therefore suggest that cationic and anionic salt mixtures like BaCl(2) with NH(4)I or salts like CdI, BaI where each ion has a significant anomalous signal for a given X-ray wavelength may be valuable reagents for phasing during structure determination.

PubMed: 22562242
DOI: 10.1007/s10969-012-9137-3

実験手法

X-RAY DIFFRACTION (2.4 Å)