3VCB

C425S mutant of the C-terminal cytoplasmic domain of non-structural protein 4 from mouse hepatitis virus A59

3VCB の概要

• エントリーDOI: 10.2210/pdb3vcb/pdb
• 分子名称: RNA-directed RNA polymerase (2 entities in total)
• 機能のキーワード: new fold; homodimer, host membrane; multi-pass membrane protein, cytoplasmic, hydrolase, viral protein
• 由来する生物種: Murine hepatitis virus
• 細胞内の位置: Host cytoplasm, host perinuclear region (By similarity). Host membrane; Multi-pass membrane protein (By similarity): Q9J3E9
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21169.71

構造登録者

Xu, X.,Lou, Z.,Ma, Y.,Chen, X.,Yang, Z.,Tong, X.,Zhao, Q.,Xu, Y.,Deng, H.,Bartlam, M.,Rao, Z. (登録日: 2012-01-03, 公開日: 2012-01-11, 最終更新日: 2024-02-28)

主引用文献

Xu, X.,Lou, Z.,Ma, Y.,Chen, X.,Yang, Z.,Tong, X.,Zhao, Q.,Xu, Y.,Deng, H.,Bartlam, M.,Rao, Z.
Crystal structure of the C-terminal cytoplasmic domain of non-structural protein 4 from mouse hepatitis virus A59.
Plos One, 4:e6217-e6217, 2009

PubMed Abstract: The replication of coronaviruses takes place on cytoplasmic double membrane vesicles (DMVs) originating in the endoplasmic reticulum (ER). Three trans-membrane non-structural proteins, nsp3, nsp4 and nsp6, are understood to be membrane anchors of the coronavirus replication complex. Nsp4 is localized to the ER membrane when expressed alone but is recruited into the replication complex in infected cells. It is revealed to contain four trans-membrane regions and its N- and C-termini are exposed to the cytosol.

PubMed: 19593433
DOI: 10.1371/journal.pone.0006217

実験手法

X-RAY DIFFRACTION (2.4 Å)