3VBF

Crystal structure of formaldehyde treated human Enterovirus 71 (space group I23)

3VBF の概要

• エントリーDOI: 10.2210/pdb3vbf/pdb
• 関連するPDBエントリー: 3VBH 3VBO 3VBR 3VBS 3VBU
• 分子名称: Genome Polyprotein, capsid protein VP1, Genome Polyprotein, capsid protein VP2, Genome Polyprotein, capsid protein VP3, ... (10 entities in total)
• 機能のキーワード: virus, hand-foot-and-mouth disease, enterovirus uncoating, pocket factor, adaptor-sensor, icosahedral virus
• 由来する生物種: Human enterovirus 71; 詳細
• 細胞内の位置: Picornain 3C: Host cytoplasm (By similarity). Protein 3B: Virion (By similarity): B2ZUN0 B2ZUN0 B2ZUN0 B2ZUN0
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 93234.66

構造登録者

Wang, X.,Peng, W.,Ren, J.,Hu, Z.,Xu, J.,Lou, Z.,Li, X.,Yin, W.,Shen, X.,Porta, C.,Walter, T.S.,Evans, G.,Axford, D.,Owen, R.,Rowlands, D.J.,Wang, J.,Stuart, D.I.,Fry, E.E.,Rao, Z. (登録日: 2012-01-02, 公開日: 2012-02-29, 最終更新日: 2023-09-13)

主引用文献

Wang, X.,Peng, W.,Ren, J.,Hu, Z.,Xu, J.,Lou, Z.,Li, X.,Yin, W.,Shen, X.,Porta, C.,Walter, T.S.,Evans, G.,Axford, D.,Owen, R.,Rowlands, D.J.,Wang, J.,Stuart, D.I.,Fry, E.E.,Rao, Z.
A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71.
Nat.Struct.Mol.Biol., 19:424-429, 2012

PubMed Abstract: Enterovirus 71 (EV71) is a major agent of hand, foot and mouth disease in children that can cause severe central nervous system disease and death. No vaccine or antiviral therapy is available. High-resolution structural analysis of the mature virus and natural empty particles shows that the mature virus is structurally similar to other enteroviruses. In contrast, the empty particles are markedly expanded and resemble elusive enterovirus-uncoating intermediates not previously characterized in atomic detail. Hydrophobic pockets in the EV71 capsid are collapsed in this expanded particle, providing a detailed explanation of the mechanism for receptor-binding triggered virus uncoating. These structures provide a model for enterovirus uncoating in which the VP1 GH loop acts as an adaptor-sensor for cellular receptor attachment, converting heterologous inputs to a generic uncoating mechanism, highlighting new opportunities for therapeutic intervention.

PubMed: 22388738
DOI: 10.1038/nsmb.2255

実験手法

X-RAY DIFFRACTION (2.6 Å)