3V5S
Structure of Sodium/Calcium Exchanger from Methanococcus jannaschii
Summary for 3V5S
Entry DOI | 10.2210/pdb3v5s/pdb |
Related | 3V5U |
Descriptor | Sodium/Calcium Exchanger, CADMIUM ION (2 entities in total) |
Functional Keywords | protein-cation complex, sodium/calcium exchanger, transmembrane proteins, alpha-helical, cation binding, calcium transport, metal transport |
Biological source | Methanocaldococcus jannaschii |
Cellular location | Cell membrane; Multi-pass membrane protein (Probable): Q57556 |
Total number of polymer chains | 1 |
Total formula weight | 34465.40 |
Authors | Liao, J.,Li, H.,Zeng, W.,Sauer, D.B.,Belmares, R.,Jiang, Y. (deposition date: 2011-12-16, release date: 2012-02-22, Last modification date: 2023-09-13) |
Primary citation | Liao, J.,Li, H.,Zeng, W.,Sauer, D.B.,Belmares, R.,Jiang, Y. Structural insight into the ion-exchange mechanism of the sodium/calcium exchanger. Science, 335:686-690, 2012 Cited by PubMed Abstract: Sodium/calcium (Na(+)/Ca(2+)) exchangers (NCX) are membrane transporters that play an essential role in maintaining the homeostasis of cytosolic Ca(2+) for cell signaling. We demonstrated the Na(+)/Ca(2+)-exchange function of an NCX from Methanococcus jannaschii (NCX_Mj) and report its 1.9 angstrom crystal structure in an outward-facing conformation. Containing 10 transmembrane helices, the two halves of NCX_Mj share a similar structure with opposite orientation. Four ion-binding sites cluster at the center of the protein: one specific for Ca(2+) and three that likely bind Na(+). Two passageways allow for Na(+) and Ca(2+) access to the central ion-binding sites from the extracellular side. Based on the symmetry of NCX_Mj and its ability to catalyze bidirectional ion-exchange reactions, we propose a structure model for the inward-facing NCX_Mj. PubMed: 22323814DOI: 10.1126/science.1215759 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3.5 Å) |
Structure validation
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