3V3C

Crystal Structure of Chloroplast ATP synthase c-ring from Pisum sativum

3V3C の概要

• エントリーDOI: 10.2210/pdb3v3c/pdb
• 分子名称: ATP synthase subunit c, chloroplastic, DIGALACTOSYL DIACYL GLYCEROL (DGDG), YTTRIUM (III) ION, ... (4 entities in total)
• 機能のキーワード: c-ring, proton translocation, photosynthesis, proton binding
• 由来する生物種: Pisum sativum (garden pea,peas)
• 細胞内の位置: Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein: P08212
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 111221.68

構造登録者

Saroussi, S.,Nelson, N. (登録日: 2011-12-13, 公開日: 2012-10-03, 最終更新日: 2024-02-28)

主引用文献

Saroussi, S.,Schushan, M.,Ben-Tal, N.,Junge, W.,Nelson, N.
Structure and flexibility of the C-ring in the electromotor of rotary F(o)F(1)-ATPase of pea chloroplasts.
Plos One, 7:e43045-e43045, 2012

PubMed Abstract: A ring of 8-15 identical c-subunits is essential for ion-translocation by the rotary electromotor of the ubiquitous F(O)F(1)-ATPase. Here we present the crystal structure at 3.4Å resolution of the c-ring from chloroplasts of a higher plant (Pisum sativum), determined using a native preparation. The crystal structure was found to resemble that of an (ancestral) cyanobacterium. Using elastic network modeling to investigate the ring's eigen-modes, we found five dominant modes of motion that fell into three classes. They revealed the following deformations of the ring: (I) ellipsoidal, (II) opposite twisting of the luminal circular surface of the ring against the stromal surface, and (III) kinking of the hairpin-shaped monomers in the middle, resulting in bending/stretching of the ring. Extension of the elastic network analysis to rings of different c(n)-symmetry revealed the same classes of dominant modes as in P. sativum (c(14)). We suggest the following functional roles for these classes: The first and third classes of modes affect the interaction of the c-ring with its counterparts in F(O), namely subunits a and bb'. These modes are likely to be involved in ion-translocation and torque generation. The second class of deformation, along with deformations of subunits γ and ε might serve to elastically buffer the torque transmission between F(O) and F(1).

PubMed: 23049735
DOI: 10.1371/journal.pone.0043045

実験手法

X-RAY DIFFRACTION (3.402 Å)