3V21

Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA

3V21 の概要

• エントリーDOI: 10.2210/pdb3v21/pdb
• 関連するPDBエントリー: 3v1z 3v20
• 分子名称: Endonuclease Bse634IR, DNA (5'-D(*TP*TP*CP*GP*AP*CP*CP*GP*GP*TP*CP*GP*A)-3') (3 entities in total)
• 機能のキーワード: restriction endonuclease, protein-dna complex, hydrolase, dna binding protein, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 325648.25

構造登録者

Manakova, E.N.,Grazulis, S.,Golovenko, D.,Tamulaitiene, G. (登録日: 2011-12-11, 公開日: 2012-04-25, 最終更新日: 2023-09-13)

主引用文献

Manakova, E.,Grazulis, S.,Zaremba, M.,Tamulaitiene, G.,Golovenko, D.,Siksnys, V.
Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease.
Nucleic Acids Res., 40:6741-6751, 2012

PubMed Abstract: Restriction endonuclease Bse634I recognizes and cleaves the degenerate DNA sequence 5'-R/CCGGY-3' (R stands for A or G; Y for T or C, '/' indicates a cleavage position). Here, we report the crystal structures of the Bse634I R226A mutant complexed with cognate oligoduplexes containing ACCGGT and GCCGGC sites, respectively. In the crystal, all potential H-bond donor and acceptor atoms on the base edges of the conserved CCGG core are engaged in the interactions with Bse634I amino acid residues located on the α6 helix. In contrast, direct contacts between the protein and outer base pairs are limited to van der Waals contact between the purine nucleobase and Pro203 residue in the major groove and a single H-bond between the O2 atom of the outer pyrimidine and the side chain of the Asn73 residue in the minor groove. Structural data coupled with biochemical experiments suggest that both van der Waals interactions and indirect readout contribute to the discrimination of the degenerate base pair by Bse634I. Structure comparison between related enzymes Bse634I (R/CCGGY), NgoMIV (G/CCGGC) and SgrAI (CR/CCGGYG) reveals how different specificities are achieved within a conserved structural core.

PubMed: 22495930
DOI: 10.1093/nar/gks300

実験手法

X-RAY DIFFRACTION (2.7 Å)