3V1V

Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and geranyl-S-thiolodiphosphate

3V1V の概要

• エントリーDOI: 10.2210/pdb3v1v/pdb
• 関連するPDBエントリー: 3V1X
• 分子名称: 2-methylisoborneol synthase, MAGNESIUM ION, GERANYL S-THIOLODIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: class i terpenoid cyclase fold, ddxxxxd motif, ndxxsxxxe motif, 2-methylisoborneol biosynthesis, biosynthesis of 2-methylisoborneol, lyase
• 由来する生物種: Streptomyces coelicolor
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47865.85

構造登録者

Koksal, M.,Christianson, D.W. (登録日: 2011-12-10, 公開日: 2012-04-11, 最終更新日: 2024-02-28)

主引用文献

Koksal, M.,Chou, W.K.,Cane, D.E.,Christianson, D.W.
Structure of 2-Methylisoborneol Synthase from Streptomyces coelicolor and Implications for the Cyclization of a Noncanonical C-Methylated Monoterpenoid Substrate.
Biochemistry, 51:3011-3020, 2012

PubMed Abstract: The crystal structure of 2-methylisoborneol synthase (MIBS) from Streptomyces coelicolor A3(2) has been determined in complex with substrate analogues geranyl-S-thiolodiphosphate and 2-fluorogeranyl diphosphate at 1.80 and 1.95 Å resolution, respectively. This terpenoid cyclase catalyzes the cyclization of the naturally occurring, noncanonical C-methylated isoprenoid substrate, 2-methylgeranyl diphosphate, to form the bicyclic product 2-methylisoborneol, a volatile C(11) homoterpene alcohol with an earthy, musty odor. While MIBS adopts the tertiary structure of a class I terpenoid cyclase, its dimeric quaternary structure differs from that previously observed in dimeric terpenoid cyclases from plants and fungi. The quaternary structure of MIBS is nonetheless similar in some respects to that of dimeric farnesyl diphosphate synthase, which is not a cyclase. The structures of MIBS complexed with substrate analogues provide insights regarding differences in the catalytic mechanism of MIBS and the mechanisms of (+)-bornyl diphosphate synthase and endo-fenchol synthase, plant cyclases that convert geranyl diphosphate into products with closely related bicyclic bornyl skeletons, but distinct structures and stereochemistries.

PubMed: 22455514
DOI: 10.1021/bi201827a

実験手法

X-RAY DIFFRACTION (1.8 Å)