3UMF

Schistosoma mansoni adenylate kinase

3UMF の概要

• エントリーDOI: 10.2210/pdb3umf/pdb
• 分子名称: Adenylate kinase (2 entities in total)
• 機能のキーワード: rossmann fold, transferase
• 由来する生物種: Schistosoma mansoni (Blood fluke)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24511.40

構造登録者

Marques, I.,DeMarco, R.,Pereira, H.M. (登録日: 2011-11-13, 公開日: 2012-09-26, 最終更新日: 2023-09-13)

主引用文献

Marques, I.,Romanello, L.,Demarco, R.,Pereira, H.D.
Structural and kinetic studies of Schistosoma mansoni adenylate kinases.
Mol.Biochem.Parasitol., 185:157-160, 2012

PubMed Abstract: The human parasite Schistosoma mansoni is totally dependent on the purine salvage pathway in order to supply large quantities of purine precursors for its energy and DNA biosynthetic needs. Adenylate kinase (ADK) is responsible for the conversion of AMP (produced by the adenosine kinase reaction) into ADP, which is subsequently converted into ATP by nucleoside diphosphate kinase (NDPK). ADK and NDPK are the most active enzymes of the pathway, probably reflecting an evolutionary adaptation due to the intense use of the branch in which they participate. However, notwithstanding their importance very little information has been accumulated found regarding these enzymes. In this work two adenylate kinases from S. mansoni were cloned and heterologously expressed in Escherichia coli. The purified products were utilized in activity assays, and displayed kinetic parameters similar to the corresponding human orthologous proteins. The cytosolic S. mansoni ADK was crystallized and its structure solved allowing us to detect a difference in the nucleotide binding site when compared with the human ortholog.

PubMed: 22841753
DOI: 10.1016/j.molbiopara.2012.07.003

実験手法

X-RAY DIFFRACTION (2.047 Å)