3ULF

The light state structure of the blue-light photoreceptor Aureochrome1 LOV

3ULF の概要

• エントリーDOI: 10.2210/pdb3ulf/pdb
• 関連するPDBエントリー: 3UE6
• 分子名称: Aureochrome1, FLAVIN MONONUCLEOTIDE, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: pas/lov domain, fmn-binding blue-light photoreceptor, signaling protein
• 由来する生物種: Vaucheria frigida
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 116239.25

構造登録者

Mitra, D.,Yang, X.,Moffat, K. (登録日: 2011-11-10, 公開日: 2012-04-11, 最終更新日: 2024-11-06)

主引用文献

Mitra, D.,Yang, X.,Moffat, K.
Crystal structures of Aureochrome1 LOV suggest new design strategies for optogenetics.
Structure, 20:698-706, 2012

PubMed Abstract: Aureochrome1, a signaling photoreceptor from a eukaryotic photosynthetic stramenopile, confers blue-light-regulated DNA binding on the organism. Its topology, in which a C-terminal LOV sensor domain is linked to an N-terminal DNA-binding bZIP effector domain, contrasts with the reverse sensor-effector topology in most other known LOV-photoreceptors. How, then, is signal transmitted in Aureochrome1? The dark- and light-state crystal structures of Aureochrome1 LOV domain (AuLOV) show that its helical N- and C-terminal flanking regions are packed against the external surface of the core β sheet, opposite to the FMN chromophore on the internal surface. Light-induced conformational changes occur in the quaternary structure of the AuLOV dimer and in Phe298 of the Hβ strand in the core. The properties of AuLOV extend the applicability of LOV domains as versatile design modules that permit fusion to effector domains via either the N- or C-termini to confer blue-light sensitivity.

PubMed: 22483116
DOI: 10.1016/j.str.2012.02.016

実験手法

X-RAY DIFFRACTION (2.9 Å)