3UJK

Crystal structure of protein phosphatase ABI2

3UJK の概要

• エントリーDOI: 10.2210/pdb3ujk/pdb
• 関連するPDBエントリー: 3UJG 3UJL
• 分子名称: Protein phosphatase 2C 77, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: abi2, protein phosphatase 2c, aba signaling, hydrolase
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress,thale-cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35856.90

構造登録者

Zhou, X.E.,Soon, F.-F.,Ng, L.-M.,Kovach, A.,Tan, M.H.E.,Suino-Powell, K.M.,He, Y.,Xu, Y.,Brunzelle, J.S.,Li, J.,Melcher, K.,Xu, H.E. (登録日: 2011-11-07, 公開日: 2012-02-15, 最終更新日: 2024-02-28)

主引用文献

Soon, F.F.,Ng, L.M.,Zhou, X.E.,West, G.M.,Kovach, A.,Tan, M.H.,Suino-Powell, K.M.,He, Y.,Xu, Y.,Chalmers, M.J.,Brunzelle, J.S.,Zhang, H.,Yang, H.,Jiang, H.,Li, J.,Yong, E.L.,Cutler, S.,Zhu, J.K.,Griffin, P.R.,Melcher, K.,Xu, H.E.
Molecular mimicry regulates ABA signaling by SnRK2 kinases and PP2C phosphatases.
Science, 335:85-88, 2012

PubMed Abstract: Abscisic acid (ABA) is an essential hormone for plants to survive environmental stresses. At the center of the ABA signaling network is a subfamily of type 2C protein phosphatases (PP2Cs), which form exclusive interactions with ABA receptors and subfamily 2 Snfl-related kinase (SnRK2s). Here, we report a SnRK2-PP2C complex structure, which reveals marked similarity in PP2C recognition by SnRK2 and ABA receptors. In the complex, the kinase activation loop docks into the active site of PP2C, while the conserved ABA-sensing tryptophan of PP2C inserts into the kinase catalytic cleft, thus mimicking receptor-PP2C interactions. These structural results provide a simple mechanism that directly couples ABA binding to SnRK2 kinase activation and highlight a new paradigm of kinase-phosphatase regulation through mutual packing of their catalytic sites.

PubMed: 22116026
DOI: 10.1126/science.1215106

実験手法

X-RAY DIFFRACTION (1.9 Å)