3UIT

Overall structure of Patj/Pals1/Mals complex

Summary for 3UIT

• Entry DOI: 10.2210/pdb3uit/pdb
• Descriptor: InaD-like protein, MAGUK p55 subfamily member 5, Protein lin-7 homolog B, ACETATE ION (3 entities in total)
• Functional Keywords: l27 domain, cell polarization, cell adhesion
• Biological source: Mus musculus (mouse, human, rat); More
• Cellular location: Cell membrane ; Peripheral membrane protein : Q9Z252
• Total number of polymer chains: 4
• Total formula weight: 120605.72

Authors

Zhang, J.,Yang, X.,Long, J.,Shen, Y. (deposition date: 2011-11-06, release date: 2012-02-22, Last modification date: 2024-03-20)

Primary citation

Zhang, J.,Yang, X.,Wang, Z.,Zhou, H.,Xie, X.,Shen, Y.,Long, J.
Structure of an L27 domain heterotrimer from cell polarity complex Patj/Pals1/Mals2 reveals mutually independent L27 domain assembly mode
J.Biol.Chem., 287:11132-11140, 2012

PubMed Abstract: The assembly of supramolecular complexes in multidomain scaffold proteins is crucial for the control of cell polarity. The scaffold protein of protein associated with Lin-7 1 (Pals1) forms a complex with two other scaffold proteins, Pals-associated tight junction protein (Patj) and mammalian homolog-2 of Lin-7 (Mals2), through its tandem Lin-2 and Lin-7 (L27) domains to regulate apical-basal polarity. Here, we report the crystal structure of a 4-L27 domain-containing heterotrimer derived from the tripartite complex Patj/Pals1/Mals2. The heterotrimer consists of two cognate pairs of heterodimeric L27 domains with similar conformations. Structural analysis and biochemical data further show that the dimers assemble mutually independently. Additionally, such mutually independent assembly of the two heterodimers can be observed in another tripartite complex, Disks large homolog 1 (DLG1)/calcium-calmodulin-dependent serine protein kinase (CASK)/Mals2. Our results reveal a novel mechanism for tandem L27 domain-mediated, supramolecular complex assembly with a mutually independent mode.

PubMed: 22337881
DOI: 10.1074/jbc.M111.321216

Experimental method

X-RAY DIFFRACTION (2.05 Å)