3UID

Crystal Structure of Protein Ms6760 from Mycobacterium smegmatis

3UID の概要

• エントリーDOI: 10.2210/pdb3uid/pdb
• 分子名称: Putative uncharacterized protein (2 entities in total)
• 機能のキーワード: uncharacterized protein, srpbcc superfamily, beta sandwich, unknown function, structural genomics, tb structural genomics consortium, tbsgc
• 由来する生物種: Mycobacterium smegmatis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37134.78

構造登録者

Bajaj, R.A.,Miallau, L.,Cascio, D.,Arbing, M.,Eisenberg, D.,TB Structural Genomics Consortium (TBSGC) (登録日: 2011-11-04, 公開日: 2011-11-23, 最終更新日: 2023-09-13)

主引用文献

Bajaj, R.A.,Arbing, M.A.,Shin, A.,Cascio, D.,Miallau, L.
Crystal structure of the toxin Msmeg_6760, the structural homolog of Mycobacterium tuberculosis Rv2035, a novel type II toxin involved in the hypoxic response.
Acta Crystallogr F Struct Biol Commun, 72:863-869, 2016

PubMed Abstract: The structure of Msmeg_6760, a protein of unknown function, has been determined. Biochemical and bioinformatics analyses determined that Msmeg_6760 interacts with a protein encoded in the same operon, Msmeg_6762, and predicted that the operon is a toxin-antitoxin (TA) system. Structural comparison of Msmeg_6760 with proteins of known function suggests that Msmeg_6760 binds a hydrophobic ligand in a buried cavity lined by large hydrophobic residues. Access to this cavity could be controlled by a gate-latch mechanism. The function of the Msmeg_6760 toxin is unknown, but structure-based predictions revealed that Msmeg_6760 and Msmeg_6762 are homologous to Rv2034 and Rv2035, a predicted novel TA system involved in Mycobacterium tuberculosis latency during macrophage infection. The Msmeg_6760 toxin fold has not been previously described for bacterial toxins and its unique structural features suggest that toxin activation is likely to be mediated by a novel mechanism.

PubMed: 27917833
DOI: 10.1107/S2053230X16017957

実験手法

X-RAY DIFFRACTION (1.571 Å)